Williams S R, Goddard J M, Martin D W
Nucleic Acids Res. 1984 Jul 25;12(14):5779-87. doi: 10.1093/nar/12.14.5779.
The isolation of a cDNA clone containing the complete coding region for human purine nucleoside phosphorylase (PNP) has been described previously. In this report we present the nucleotide sequence of this cDNA clone and compare the derived amino acid sequence, encoding a protein of 32 kilodaltons, with the published amino acid composition. Using a fragment of the cDNA clone as a probe, human PNP genomic clones from a bacteriophage lambda library have been isolated and the structural organization of the wild type PNP gene determined.
先前已有关于包含人嘌呤核苷磷酸化酶（PNP）完整编码区的cDNA克隆的分离报道。在本报告中，我们给出了该cDNA克隆的核苷酸序列，并将推导的编码32千道尔顿蛋白质的氨基酸序列与已发表的氨基酸组成进行比较。利用该cDNA克隆的一个片段作为探针，从噬菌体λ文库中分离出了人PNP基因组克隆，并确定了野生型PNP基因的结构组织。
