Kimball P M, Brattain M G, Pitts A M
Br J Cancer. 1978 Jun;37(6):1015-9. doi: 10.1038/bjc.1978.147.
Cell suspensions from 5 human colonic carcinomas were fractionated by velocity sedimentation and plated in soft agar. Cluster formation was restricted to the purest fraction of epithelial cells, as had been determined by immuno- and histochemical criteria. Plating efficiencies for the 5 specimens were 1.0-4.5%. The effects of varying the incubation period and inoculum size upon growth were studied using unseparated cell suspensions from 6 specimens. Clusters were apparent after 3 weeks in culture, and maximum cluster formation was typically seen by 5 weeks. Cluster formation appeared concentration-dependent, and individual specimens varied with respect to the inoculum most conducive to growth. The maximum plating efficiencies for unseparated cells were unseparated cells were 0.4-1.7%.
对来自5个人类结肠癌的细胞悬液进行速度沉降分级，并接种于软琼脂中。如通过免疫和组织化学标准所确定的，集落形成仅限于最纯的上皮细胞部分。5个标本的接种效率为1.0 - 4.5%。使用来自6个标本的未分离细胞悬液研究了改变培养时间和接种量对生长的影响。培养3周后可见集落，通常在5周时可见最大集落形成。集落形成呈浓度依赖性，且不同标本在最有利于生长的接种量方面存在差异。未分离细胞的最大接种效率为0.4 - 1.7%。
