Meyer S, Brunner H
Zentralbl Bakteriol A. 1981 Feb;248(4):469-78.
An enzyme-immuno-assay (EIA) was developed for the determination of antibodies to Listeria monocytogenes O- and H-antigens of serotypes 1 and 4b, which are predominant in the Federal Republic of Germany. The results correlated well with the tube agglutination but the titers in EIA were considerably higher. Thus one advantage of EIA was its sensitivity. In addition, the EIA offers the possibility to detect antibodies belonging to different immunoglobulin classes. The EIA is easy to perform and required smaller amounts of reagents than the tube agglutination. Since optical densitys is determined in the EIA, the data obtained in this system are less variable than the tube agglutination which is read by eye. The method proved also to be useful for the detection of Listeria-Antigen. Approximately 0.3 microgram/ml of H-antigen could still be detected in the EIA. Since the serological diagnosis of Listeria-infections is hampered by the cross-reactions to various other bacterial antigens the detection of Listeria-antigen by a sensitive immunological procedure could be of considerable value. This is especially the case when culture procedures are not promising, e.g. during or after antimicrobial chemotherapy.
开发了一种酶免疫测定法（EIA），用于检测单核细胞增生李斯特菌血清型1和4b的O抗原和H抗原的抗体，这些血清型在德意志联邦共和国占主导地位。结果与试管凝集法相关性良好，但EIA中的效价要高得多。因此，EIA的一个优点是其灵敏度。此外，EIA还提供了检测属于不同免疫球蛋白类别的抗体的可能性。EIA易于操作，所需试剂比试管凝集法少。由于EIA中测定的是光密度，该系统获得的数据比通过肉眼读取的试管凝集法更稳定。该方法也被证明对检测李斯特菌抗原有用。在EIA中仍可检测到约0.3微克/毫升的H抗原。由于对各种其他细菌抗原的交叉反应阻碍了李斯特菌感染的血清学诊断，通过灵敏的免疫程序检测李斯特菌抗原可能具有相当大的价值。在培养程序不太有希望的情况下，例如在抗菌化疗期间或之后，尤其如此。
