Garcia A, Sierra M F, Friberg J
Fertil Steril. 1981 May;35(5):549-51. doi: 10.1016/s0015-0282(16)45499-3.
Thirty-four semen samples from fertile men were subjected to bacteriologic evaluation before and after freezing to -196 degrees C using 10% glycerol as the protective medium. Cultures of the fresh portions from all ejaculates examined showed the presence of aerobic bacteria. Staphylococcus epidermidis, Corynebacterium sp., alpha-streptococci, and nonhemolytic streptococci were found so commonly that they were considered commensals. Enterococci, Escherichia coli, S. aureus, and beta-hemolytic streptococci group B, as well as Pseudomonas aeruginosa, P. maltophilia, and P. fluorescens, were also recovered from a few semen samples of these fertile men. Practically all bacteria isolated from the fresh ejaculate were also recovered from the portions frozen in liquid nitrogen.
对34份来自生育能力正常男性的精液样本，以10%甘油作为保护介质，在冷冻至-196℃之前和之后进行了细菌学评估。对所有检测射精样本的新鲜部分进行培养，结果显示存在需氧菌。表皮葡萄球菌、棒状杆菌属、α-链球菌和非溶血性链球菌极为常见，因此被视为共生菌。粪肠球菌、大肠杆菌、金黄色葡萄球菌、B组β-溶血性链球菌，以及铜绿假单胞菌、嗜麦芽窄食单胞菌和荧光假单胞菌，也从这些生育能力正常男性的一些精液样本中分离出来。实际上，从新鲜射精样本中分离出的所有细菌在液氮冷冻的部分中也能找到。
