Sapezhinskiĭ I I, Dontsova E G
Biofizika. 1978 Jul-Aug;23(4):583-8.
Effect of dyes of different classes on characteristics of X-chemoluminescence of human serum albumin solutions is studied. Intensification of lumininescence by the addition of uranin, eosin, tetrachlorfluorescein and erythrozan was found. The process of luminescence intensification with the addition of uranine was thoroughly studied (kinetic regularities, spectra, concentration relationships). It has been shown that kinetic regularities specify mainily the rapid component of X-chemoluminescence, that the initiation of chemoluminescence is related to the reactions of protein groups with hydroxyl radicals, that the intensification of luminescence proceeds in the protein-dye complex, that light emission of activated chemoluminescence took place from the singlet levels of the bound dye.
研究了不同种类染料对人血清白蛋白溶液X-化学发光特性的影响。发现添加荧光素钠、曙红、四氯荧光素和赤藓红可增强发光。对添加荧光素钠后发光增强的过程进行了深入研究（动力学规律、光谱、浓度关系）。结果表明，动力学规律主要决定了X-化学发光的快速成分，化学发光的引发与蛋白质基团与羟基自由基的反应有关，发光增强发生在蛋白质-染料复合物中，活化化学发光的光发射源于结合染料的单重态能级。
