Dobretsov G E, Petrov V A, Vladimirov Iu A
Biofizika. 1978 Jul-Aug;23(4):629-32.
A scheme of the location of a fluorescent probe 4-dimethylaminochalcone (DCM) in a phospholipid membrane has been proposed. According to the scheme the DMC dimethylamino group is located near phosphate groups and the oxygen atom--in the carbonyl groups region of phosphatidylcholine molecules. It is shown that DMC fluorescence is dynamically quenched by water molecules in the membrane. Therefore the quantum yield and, partly the position of DMC fluorescence maximum are determined by the water concentration near the probe, by the ability of water molecules to penetrate into the membrane as far as the carbonyl groups of fatty acid residues and by the water mobility in the membrane layer.
提出了荧光探针4-二甲基氨基查耳酮（DCM）在磷脂膜中位置的示意图。根据该示意图，DMC的二甲氨基位于磷酸基团附近，而氧原子位于磷脂酰胆碱分子的羰基区域。结果表明，膜中的水分子会动态猝灭DMC的荧光。因此，量子产率以及部分DMC荧光最大值的位置取决于探针附近的水浓度、水分子渗透到膜中直至脂肪酸残基羰基的能力以及水分子在膜层中的流动性。
