Shishlov Iu V, Nenashev V A
Biofizika. 1978 Jul-Aug;23(4):633-7.
The floating membrane vesicle is fixed by the counter solution flow in different points along the radius of a cylinder electrophoretic chamber, which permits to measure the vesicle electrophoretic mobility (EM). Close state condition of the chamber is provided for by the capillary system successively connected with the chamber. Relationship between EM of bimolecular lipid membranes (BLM) and pH and ionic concentration of aqueous solution qualitatively coincides with similar relationship for liposomes. The EM value of BLM essentially decreases in solution containing polyene antibiotics nystatine and levorin when derivative of cholesterol having 3betaOH-groups is present in the membrane.
浮动膜泡通过在圆柱电泳室半径上不同点处的反向溶液流固定,这使得能够测量膜泡的电泳迁移率(EM)。电泳室的封闭状态由与电泳室相继相连的毛细管系统提供。双分子脂膜(BLM)的EM与水溶液的pH值和离子浓度之间的关系在性质上与脂质体的类似关系相符。当膜中存在具有3βOH基团的胆固醇衍生物时,BLM在含有多烯抗生素制霉菌素和左霉素的溶液中的EM值会显著降低。