Guldvog I, Gedde-Dahl D, Berstad A
Scand J Gastroenterol. 1980;15(8):939-48. doi: 10.3109/00365528009181795.
The present studies were performed on a double-pouch dog with one vagally innervated Amdrup pouch (AP) and one denervated Heidenhain pouch (HP), allowing comparison of pepsin secretion from innervated and denervated mucosa at the same time in the same animal. 'Non-active' secretion of pepsin was determined by instillation in the pouches of 0.1 M and 0.005 M HCl, 0.15 M NaCl, and 0.03 M phosphate buffer, and well-known stimulators of gastric secretion such as histamine, pentagastrin, bethanechol, and 2-deoxy-D-glucose (2-DG) were tested as pepsigogues. Cholinergic stimulation by 2-DG and bethanechol (Urecholine) was clearly the most potent stimulus of pepsin secretion. 2-DG elicited secretion only from innervated mucosa, whereas the responses to bethanechol were similar in the two pouches. Histamine and pentagastrin were weak stimulators, but both provoked active and sustained secretion of pepsin when given in small doses. Higher doses of histamine strongly inhibited pepsin output. The effects of histamine were independent of vagal innervation. By contrast, the active stimulation by pentagastrin only took place in innervated mucosa. The highest outputs were seen in the lowest doses, but also very high doses of pentagastrin elicited active pepsin secretion in the AP. Medium doses of pentagastrin brought the secretion down the the 'non-active' level. The chief cells in the denervated mucosa were quite insensible to pentagastrin, and the pepsin output in the HP equalled the non-active' response at all dose levels.
本研究在双袋犬身上进行，该犬有一个受迷走神经支配的阿姆德鲁普袋（AP）和一个去神经支配的海登海因袋（HP），这样可以在同一动物体内同时比较受神经支配和去神经支配的黏膜中胃蛋白酶的分泌情况。通过向袋内滴注0.1 M和0.005 M盐酸、0.15 M氯化钠和0.03 M磷酸盐缓冲液来测定胃蛋白酶的“非活性”分泌，并测试了组胺、五肽胃泌素、氨甲酰甲胆碱和2-脱氧-D-葡萄糖（2-DG）等著名的胃分泌刺激剂作为促胃蛋白酶分泌剂。2-DG和氨甲酰甲胆碱（乌拉胆碱）的胆碱能刺激显然是最有效的胃蛋白酶分泌刺激剂。2-DG仅从受神经支配的黏膜引发分泌，而对氨甲酰甲胆碱的反应在两个袋中相似。组胺和五肽胃泌素是较弱的刺激剂，但小剂量给药时都会引发胃蛋白酶的活性和持续分泌。高剂量组胺强烈抑制胃蛋白酶的分泌。组胺的作用与迷走神经支配无关。相比之下，五肽胃泌素的活性刺激仅发生在受神经支配的黏膜中。最低剂量时胃蛋白酶分泌量最高，但非常高剂量的五肽胃泌素也能在AP中引发活性胃蛋白酶分泌。中等剂量的五肽胃泌素使分泌降至“非活性”水平。去神经支配黏膜中的主细胞对五肽胃泌素相当不敏感，HP中的胃蛋白酶分泌量在所有剂量水平都等于“非活性”反应。
