Guldvog I, Gedde-Dahl D
Scand J Gastroenterol. 1980;15(8):929-37. doi: 10.3109/00365528009181794.
The double-pouch dog with one vagally innervated Amdrup pouch (AP) taken from the lesser curvature side of the stomach and one denervated Heidenhain pouch (HP) from the corporo-fundic part permitted comparison of gastric secretion from innervated and denervated mucosa at the same time in the same animal. Food stimulation with a mixture of liver, heart, and bonemeal showed significantly higher response from AP than from HP for all doses, and the difference between the pouches in total acid output was constant for all doses, indicating that the relative contribution of vagal innervation is diminished when the size of the protein meal is increased. The difference between innervated and denervated mucosa is achieved by an immediate high response from the AP. After 150 min the responses to the larger meals were identical in the two pouches. Acid output during stimulation with pentagastrin reached 64.4% of maximal response to histamine in the AP and 36.2% in the HP, the innervated mucosa giving a statistically significant higher response for all doses. Absence of innervation gave an HP response similar to non-competitive inhibition with significantly lower calculated maximal response and a CD50 not different from the CD50 for the AP. Twenty-four hour secretion studies during optimal physiological conditions with the dog fed two daily meals of standard composition showed sparse secretion from denervated mucosa, whereas a great part of the capacity of innervated mucosa is utilized during daily conditions.
双袋犬模型中，一个是取自胃小弯侧的迷走神经支配的阿姆德鲁普袋（AP），另一个是取自胃体-胃底部的去神经海登海因袋（HP），这使得在同一只动物身上能够同时比较来自神经支配和去神经支配黏膜的胃液分泌情况。用肝脏、心脏和骨粉混合物进行食物刺激时，所有剂量下AP的反应均显著高于HP，且各剂量下两个袋之间的总酸分泌差异恒定，这表明当蛋白餐量增加时，迷走神经支配的相对作用减弱。神经支配和去神经支配黏膜之间的差异是通过AP立即产生的高反应实现的。150分钟后，两个袋对较大餐食的反应相同。用五肽胃泌素刺激时，AP的酸分泌量达到对组胺最大反应的64.4%，HP为36.2%，神经支配的黏膜在所有剂量下的反应在统计学上均显著更高。去神经支配使得HP的反应类似于非竞争性抑制，计算得出的最大反应显著更低，且半数有效浓度（CD50）与AP的CD50无异。在最佳生理条件下，给狗每日喂食两顿标准成分餐食进行24小时分泌研究，结果显示去神经支配的黏膜分泌稀少，而在日常情况下，神经支配的黏膜大部分分泌能力得到利用。
