一种通过注射[3H]苯丙氨酸来测量组织中蛋白质合成速率的快速便捷技术。
A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine.
作者信息
Garlick P J, McNurlan M A, Preedy V R
出版信息
Biochem J. 1980 Nov 15;192(2):719-23. doi: 10.1042/bj1920719.
Abstract
A rapid procedure for measuring the specific radioactivity of phenylalanine in tissues was developed. This facilitates the accurate determination of rates of protein synthesis in a wide range of tissues by injection of 150 mumol of L-[4-(3)H]phenylalanine/100 g body wt. The large dose of amino acid results in a rapid rise in specific radioactivity of free phenylalanine in tissues to values close to that in plasma, followed by a slow but linear fall. This enables the rate of protein synthesis to be calculated from measurements of the specific radioactivity of free and protein-bound phenylalanine in tissues during a 10 min period after injection of radioisotope.
摘要
开发了一种快速测量组织中苯丙氨酸比放射性的方法。通过注射150 μmol的L-[4-(³H)]苯丙氨酸/100 g体重，这有助于准确测定多种组织中的蛋白质合成速率。大剂量的氨基酸会导致组织中游离苯丙氨酸的比放射性迅速上升至接近血浆中的值，随后缓慢但呈线性下降。这使得在注射放射性同位素后的10分钟内，通过测量组织中游离和与蛋白质结合的苯丙氨酸的比放射性来计算蛋白质合成速率成为可能。
相似文献
1
A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine.一种通过注射[3H]苯丙氨酸来测量组织中蛋白质合成速率的快速便捷技术。
Biochem J. 1980 Nov 15;192(2):719-23. doi: 10.1042/bj1920719.
2
Stimulation of epidermal protein synthesis in vivo by topical triamcinolone acetonide.外用曲安奈德对体内表皮蛋白合成的刺激作用。
Biochem J. 1987 Nov 1;247(3):525-30. doi: 10.1042/bj2470525.
3
Phenylalanine flooding dose procedure is effective in measuring intestinal and liver protein synthesis in sheep.苯丙氨酸负荷剂量法在测量绵羊肠道和肝脏蛋白质合成方面是有效的。
J Nutr. 1992 Dec;122(12):2398-407. doi: 10.1093/jn/122.12.2398.
4
Validation of a fast, simple and reliable method to assess protein synthesis in individual tissues by intraperitoneal injection of a flooding dose of [3H]phenylalanine.通过腹腔注射过量[3H]苯丙氨酸来评估个体组织中蛋白质合成的一种快速、简单且可靠方法的验证。
J Biochem Biophys Methods. 1987 Sep;14(6):349-54. doi: 10.1016/0165-022x(87)90029-7.
5
The effect of elevated plasma phenylalanine levels on protein synthesis rates in adult rat brain.血浆苯丙氨酸水平升高对成年大鼠大脑蛋白质合成速率的影响。
Biochem J. 1994 Sep 1;302 ( Pt 2)(Pt 2):601-10. doi: 10.1042/bj3020601.
6
Measurement of protein synthesis in rat lungs perfused in situ.原位灌注大鼠肺脏中蛋白质合成的测量。
Biochem J. 1980 Apr 15;188(1):269-78. doi: 10.1042/bj1880269.
7
Validation of the flooding dose technique to determine fractional rates of protein synthesis in a model bivalve species, the blue mussel (Mytilus edulis L.).验证水淹剂量技术以确定双壳贝类模型物种蓝贻贝（Mytilus edulis L.）中蛋白质合成的分数率。
Comp Biochem Physiol A Mol Integr Physiol. 2016 Jan;191:166-173. doi: 10.1016/j.cbpa.2015.10.019. Epub 2015 Oct 23.
8
In vivo measurement of myocardial protein turnover using an indicator dilution technique.使用指示剂稀释技术对心肌蛋白周转率进行体内测量。
Circ Res. 1990 Oct;67(4):902-12. doi: 10.1161/01.res.67.4.902.
9
Whole body protein synthesis: studies with different amino acids in the rat.全身蛋白质合成：大鼠不同氨基酸的研究。
Am J Physiol. 1989 Nov;257(5 Pt 1):E639-46. doi: 10.1152/ajpendo.1989.257.5.E639.
10
A rapid and convenient method for measuring the fractional rate of protein synthesis in ectothermic animal tissues using a stable isotope tracer.一种使用稳定同位素示踪剂测量变温动物组织中蛋白质合成分数率的快速便捷方法。
Comp Biochem Physiol B Biochem Mol Biol. 2015 Apr;182:1-5. doi: 10.1016/j.cbpb.2014.11.006. Epub 2014 Dec 3.
引用本文的文献
1
Effects of a methionine deficiency on chicken tissue protein turnover: comparative analysis of methionine source.蛋氨酸缺乏对鸡组织蛋白质周转的影响：蛋氨酸来源的比较分析。
Poult Sci. 2025 Jun 7;104(9):105410. doi: 10.1016/j.psj.2025.105410.
2
Regulation of Protein Synthesis at the Translational Level: Novel Findings in Cardiovascular Biology.翻译水平上蛋白质合成的调控：心血管生物学中的新发现。
Biomolecules. 2025 May 9;15(5):692. doi: 10.3390/biom15050692.
3
Dual Translational Control in Cardiomyocytes by Heterogeneous mTORC1 and Hypertrophic ERK Activation.心肌细胞中异质性mTORC1和肥大性ERK激活的双重翻译控制
bioRxiv. 2025 Feb 11:2025.02.10.635974. doi: 10.1101/2025.02.10.635974.
4
Life in the margins: the effect of immersion/emersion and tidal cycle on the North Atlantic limpet Patella vulgata protein synthesis rates.边缘生活：沉浸/暴露和潮汐周期对北大西洋帽贝 Patella vulgata 蛋白质合成率的影响。
J Comp Physiol B. 2024 Dec;194(6):779-792. doi: 10.1007/s00360-024-01582-0. Epub 2024 Sep 11.
5
RNA-binding proteins in cardiovascular biology and disease: the beat goes on.心血管生物学和疾病中的 RNA 结合蛋白：跳动不止。
Nat Rev Cardiol. 2024 Jun;21(6):361-378. doi: 10.1038/s41569-023-00958-z. Epub 2024 Jan 2.
6
Resilience and phenotypic plasticity of Arctic char () facing cyclic hypoxia: insights into growth, energy stores and hepatic metabolism.北极红点鲑（Salvelinus alpinus）面对周期性缺氧时的恢复力和表型可塑性：对生长、能量储存和肝脏代谢的见解
Conserv Physiol. 2023 Dec 15;11(1):coad099. doi: 10.1093/conphys/coad099. eCollection 2023.
7
Evolutionary Adaptation of Protein Turnover in White Muscle of Stenothermal Antarctic Fish: Elevated Cold Compensation at Reduced Thermal Responsiveness.蛋白质周转率在耐冷南极鱼类白肌中的进化适应：在降低热反应性的情况下提高冷补偿。
Biomolecules. 2023 Oct 11;13(10):1507. doi: 10.3390/biom13101507.
8
Protein Synthesis Determined from Non-Radioactive Phenylalanine Incorporated by Antarctic Fish.通过南极鱼类摄入的非放射性苯丙氨酸测定蛋白质合成
Metabolites. 2023 Feb 24;13(3):338. doi: 10.3390/metabo13030338.
9
mRNA Metabolism and Hypertension.信使核糖核酸代谢与高血压
Biomedicines. 2023 Jan 3;11(1):118. doi: 10.3390/biomedicines11010118.
10
Loss of epigenetic information as a cause of mammalian aging.作为哺乳动物衰老原因的表观遗传信息丢失。
Cell. 2023 Jan 19;186(2):305-326.e27. doi: 10.1016/j.cell.2022.12.027. Epub 2023 Jan 12.
本文引用的文献
1
Suppression of protein turnover by amino acids in the perfused rat liver.灌注大鼠肝脏中氨基酸对蛋白质周转的抑制作用。
J Biol Chem. 1972 Oct 25;247(20):6474-81.
2
3
Some special kinetic problems of transport.传输的一些特殊动力学问题。
Adv Enzymol Relat Areas Mol Biol. 1969;32:1-20. doi: 10.1002/9780470122778.ch1.
4
Insulin in the regulation of protein turnover in heart and skeletal muscle.胰岛素在心脏和骨骼肌蛋白质周转调节中的作用。
Fed Proc. 1974 Apr;33(4):1098-1104.
5
The diurnal response of muscle and liver protein synthesis in vivo in meal-fed rats.进食大鼠体内肌肉和肝脏蛋白质合成的昼夜反应。
Biochem J. 1973 Dec;136(4):935-45. doi: 10.1042/bj1360935.
6
Leucine. A possible regulator of protein turnover in muscle.亮氨酸。一种肌肉中蛋白质周转的潜在调节因子。
J Clin Invest. 1975 Nov;56(5):1250-61. doi: 10.1172/JCI108201.
7
Effects of insulin, glucose, and amino acids on protein turnover in rat diaphragm.胰岛素、葡萄糖和氨基酸对大鼠膈肌蛋白质周转的影响。
J Biol Chem. 1975 Jan 10;250(1):290-8.
8
Tritosol: a new scintillation cocktail based on Triton X-100.三乙醇胺：一种基于曲拉通X-100的新型闪烁液。
Anal Biochem. 1975 Feb;63(2):555-8. doi: 10.1016/0003-2697(75)90379-6.
9
A method for measuring brain protein synthesis rates in young and adult rats.一种测量幼年和成年大鼠脑蛋白合成率的方法。
J Neurochem. 1975 Feb;24(2):337-44. doi: 10.1111/j.1471-4159.1975.tb11885.x.
10
A simple micro-assay for inorganic phosphate.一种用于无机磷酸盐的简单微量测定法。
Anal Biochem. 1976 Sep;75(1):201-10. doi: 10.1016/0003-2697(76)90071-3.
Zotero 插件