Carlo P, Martelli A, Finollo R, Bignone F A
Boll Soc Ital Biol Sper. 1981 Feb 28;57(4):414-20.
A sensitive viscosimetric approach for measuring DNA single-strand breaks induced by low doses of alkylating agents is presented. The assay is based on the breaks-induced increasing rate of strand separation of rat liver DNA in alkaline solution (pH 12,5; 22 C). Because of the strong dependence of viscosity on DNA unwinding, measurement of time-course of viscosity increase can be used to detect small breakage of DNA. The kinetics of DNA denaturation has been also studied under two different alkaline conditions.
本文提出了一种灵敏的粘度测定方法，用于测量低剂量烷基化剂诱导的DNA单链断裂。该测定基于碱性溶液（pH 12.5；22℃）中大鼠肝脏DNA链分离的断裂诱导增加率。由于粘度对DNA解旋的强烈依赖性，粘度增加时间进程的测量可用于检测DNA的小断裂。还在两种不同的碱性条件下研究了DNA变性的动力学。
