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在对丝裂霉素C敏感的大肠杆菌mtc突变体中,十二烷基硫酸钠敏感的隔膜形成。

Sodium dodecyl sulfate-sensitive septation in a mitomycin C-sensitive, mtc, mutant of Escherichia coli.

作者信息

Higashi T, Suzuki K, Otsuji N

出版信息

J Bacteriol. 1981 Jun;146(3):1117-23. doi: 10.1128/jb.146.3.1117-1123.1981.

DOI:10.1128/jb.146.3.1117-1123.1981
PMID:6787013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216968/
Abstract

A mitomycin C-sensitive, mtc, mutant of Escherichia coli has an altered cell surface and is sensitive to sodium dodecyl sulfate (SDS). The mutant, M27, formed multinucleate nonseptated filaments in the presence of a low concentration of SDS (50 microgram/ml). When the culture grown at that concentration of SDS was diluted with an SDS-free medium, the filaments began to divide at a very rapid rate after a lag of about 20 min. Chloramphenicol inhibited this recovery division when added within 10 min after SDS dilution but did not inhibit the division when added 20 min after dilution. Penicillin G at a low concentration, which is enough to cause filamentation, had virtually no effect on the recovery division of SDS-induced filaments. The division of penicillin G-induced filaments was inhibited by SDS.

摘要

大肠杆菌的一种对丝裂霉素C敏感的mtc突变体具有改变的细胞表面,并且对十二烷基硫酸钠(SDS)敏感。该突变体M27在低浓度SDS(50微克/毫升)存在下形成多核无隔膜丝状体。当在该SDS浓度下生长的培养物用无SDS培养基稀释时,丝状体在约20分钟的延迟后开始以非常快的速度分裂。在SDS稀释后10分钟内加入氯霉素可抑制这种恢复分裂,但在稀释后20分钟加入则不抑制分裂。低浓度的青霉素G足以引起丝状体形成,对SDS诱导的丝状体的恢复分裂几乎没有影响。SDS可抑制青霉素G诱导的丝状体的分裂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac18/216968/4fc05bfc4a06/jbacter00271-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac18/216968/4fc05bfc4a06/jbacter00271-0296-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ac18/216968/4fc05bfc4a06/jbacter00271-0296-a.jpg

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