大肠杆菌clpP和clpB突变体对十二烷基硫酸钠的超敏反应
Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli.
作者信息
Rajagopal Soumitra, Sudarsan Narasimhan, Nickerson Kenneth W
机构信息
School of Biological Sciences, University of Nebraska, Lincoln, Nebraska 68588-0666, USA.
出版信息
Appl Environ Microbiol. 2002 Aug;68(8):4117-21. doi: 10.1128/AEM.68.8.4117-4121.2002.
Abstract
We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and lon mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western immunoblot analysis increased ca. sixfold as the levels of added SDS increased from 0 to 2%. Capsular colanic acid, measured as uronic acid, increased ca. sixfold as the levels of added SDS increased from 2 to 10%. Based on these findings, 3 of the 19 previously identified SDS shock proteins (M. Adamowicz, P. M. Kelley, and K. W. Nickerson, J. Bacteriol. 173:229-233, 1991) are tentatively identified as ClpP, ClpX, and ClpB.
摘要
我们研究了大肠杆菌的clpP和clpB突变体对十二烷基硫酸钠（SDS）的超敏反应。野生型大肠杆菌MC4100和lon突变体在10% SDS存在的情况下能够生长，而同源的clpP和clpB单突变体在SDS浓度高于0.5%时无法生长，clpA和clpX单突变体在SDS浓度高于5.0%时无法生长。对于野生型大肠杆菌，通过蛋白质免疫印迹分析测定的细胞ClpP水平，随着添加的SDS水平从0%增加到2%，大约增加了六倍。以糖醛酸衡量的荚膜多糖酸，随着添加的SDS水平从2%增加到10%，大约增加了六倍。基于这些发现，在先前鉴定的19种SDS休克蛋白中（M. Adamowicz、P. M. Kelley和K. W. Nickerson，《细菌学杂志》173:229 - 233，1991），暂时确定其中3种为ClpP、ClpX和ClpB。
相似文献
1
Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli.大肠杆菌clpP和clpB突变体对十二烷基硫酸钠的超敏反应
Appl Environ Microbiol. 2002 Aug;68(8):4117-21. doi: 10.1128/AEM.68.8.4117-4121.2002.
2
ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities.ClpX，大肠杆菌ATP依赖性Clp蛋白酶的一种替代亚基。序列及体内活性。
J Biol Chem. 1993 Oct 25;268(30):22618-26.
3
Global transcriptional analysis of clpP mutations of type 2 Streptococcus pneumoniae and their effects on physiology and virulence.2型肺炎链球菌clpP突变的全转录组分析及其对生理学和毒力的影响。
J Bacteriol. 2002 Jul;184(13):3508-20. doi: 10.1128/JB.184.13.3508-3520.2002.
4
Global role for ClpP-containing proteases in stationary-phase adaptation of Escherichia coli.含ClpP蛋白酶在大肠杆菌稳定期适应中的全局作用。
J Bacteriol. 2003 Jan;185(1):115-25. doi: 10.1128/JB.185.1.115-125.2003.
5
Expression of different-size transcripts from the clpP-clpX operon of Escherichia coli during carbon deprivation.大肠杆菌clpP-clpX操纵子在碳源缺乏时不同大小转录本的表达
J Bacteriol. 2000 Dec;182(23):6630-7. doi: 10.1128/JB.182.23.6630-6637.2000.
6
Regulation of Escherichia coli starvation sigma factor (sigma s) by ClpXP protease.ClpXP蛋白酶对大肠杆菌饥饿σ因子（σs）的调控
J Bacteriol. 1996 Jan;178(2):470-6. doi: 10.1128/jb.178.2.470-476.1996.
7
Functional domains of the ClpA and ClpX molecular chaperones identified by limited proteolysis and deletion analysis.通过有限蛋白酶解和缺失分析鉴定的ClpA和ClpX分子伴侣的功能结构域。
J Biol Chem. 2001 Aug 3;276(31):29420-9. doi: 10.1074/jbc.M103489200. Epub 2001 May 9.
8
The clp proteases of Bacillus subtilis are directly involved in degradation of misfolded proteins.枯草芽孢杆菌的Clp蛋白酶直接参与错误折叠蛋白质的降解。
J Bacteriol. 2000 Jun;182(11):3259-65. doi: 10.1128/JB.182.11.3259-3265.2000.
9
Identification and transcriptional control of the genes encoding the Caulobacter crescentus ClpXP protease.新月柄杆菌ClpXP蛋白酶编码基因的鉴定与转录调控。
J Bacteriol. 1999 May;181(10):3039-50. doi: 10.1128/JB.181.10.3039-3050.1999.
10
The stroma of higher plant plastids contain ClpP and ClpC, functional homologs of Escherichia coli ClpP and ClpA: an archetypal two-component ATP-dependent protease.高等植物质体的基质含有ClpP和ClpC，它们是大肠杆菌ClpP和ClpA的功能同源物：一种典型的双组分ATP依赖性蛋白酶。
Plant Cell. 1995 Oct;7(10):1713-22. doi: 10.1105/tpc.7.10.1713.
引用本文的文献
1
The broad antibacterial activity of a small synthetic receptor for cellular phosphatidylglycerol lipids.一种针对细胞磷脂酰甘油脂质的小型合成受体具有广泛的抗菌活性。
Folia Microbiol (Praha). 2023 Jun;68(3):465-476. doi: 10.1007/s12223-022-01023-x. Epub 2023 Jan 9.
2
Atypical cyclic di-AMP signaling is essential for Porphyromonas gingivalis growth and regulation of cell envelope homeostasis and virulence.非典型环二鸟苷酸信号对于牙龈卟啉单胞菌的生长以及细胞包膜动态平衡和毒力的调控是必不可少的。
NPJ Biofilms Microbiomes. 2022 Jul 6;8(1):53. doi: 10.1038/s41522-022-00316-w.
3
Starvation induces shrinkage of the bacterial cytoplasm.饥饿会导致细菌细胞质收缩。
Proc Natl Acad Sci U S A. 2021 Jun 15;118(24). doi: 10.1073/pnas.2104686118.
4
Dietary Emulsifiers Alter Composition and Activity of the Human Gut Microbiota , Irrespective of Chemical or Natural Emulsifier Origin.膳食乳化剂会改变人体肠道微生物群的组成和活性，无论其来源是化学乳化剂还是天然乳化剂。
Front Microbiol. 2020 Nov 5;11:577474. doi: 10.3389/fmicb.2020.577474. eCollection 2020.
5
Longer Ubiquinone Side Chains Contribute to Enhanced Farnesol Resistance in Yeasts.更长的泛醌侧链有助于增强酵母对法尼醇的抗性。
Microorganisms. 2020 Oct 23;8(11):1641. doi: 10.3390/microorganisms8111641.
6
cjrABC-senB hinders survival of extraintestinal pathogenic E. coli in the bloodstream through triggering complement-mediated killing.cjrABC-senB 通过触发补体介导的杀伤作用来阻碍肠外致病性大肠杆菌在血液中的存活。
J Biomed Sci. 2020 Aug 6;27(1):86. doi: 10.1186/s12929-020-00677-4.
7
ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis.ClpP 参与粪肠球菌的应激耐受、生物膜形成、抗微生物药物耐受和毒力。
BMC Microbiol. 2020 Feb 7;20(1):30. doi: 10.1186/s12866-020-1719-9.
8
Sulfate Ester Detergent Degradation in Is Subject to both Positive and Negative Regulation.硫酸盐酯清洁剂的降解受到正向和负向调控。
Appl Environ Microbiol. 2019 Nov 14;85(23). doi: 10.1128/AEM.01352-19. Print 2019 Dec 1.
9
Genetically Programmable Self-Regenerating Bacterial Hydrogels.基因可编程的自修复细菌水凝胶。
Adv Mater. 2019 Oct;31(40):e1901826. doi: 10.1002/adma.201901826. Epub 2019 Aug 12.
10
Differential MS2 Interaction with Food Contact Surfaces Determined by Atomic Force Microscopy and Virus Recovery.通过原子力显微镜和病毒回收测定MS2与食品接触表面的差异相互作用
Appl Environ Microbiol. 2017 Dec 1;83(24). doi: 10.1128/AEM.01881-17. Print 2017 Dec 15.
本文引用的文献
1
Activity of the APC(Cdh1) form of the anaphase-promoting complex persists until S phase and prevents the premature expression of Cdc20p.后期促进复合物的APC（Cdh1）形式的活性持续到S期，并阻止Cdc20p的过早表达。
J Cell Biol. 2001 Jul 9;154(1):85-94. doi: 10.1083/jcb.200102007.
2
ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli.ClpB与DnaK、DnaJ和GrpE协同作用以抑制蛋白质聚集。一种来自大肠杆菌的新型多分子伴侣系统。
J Biol Chem. 1999 Oct 1;274(40):28083-6. doi: 10.1074/jbc.274.40.28083.
3
Here's the hook: similar substrate binding sites in the chaperone domains of Clp and Lon.关键在于：Clp和Lon的伴侣结构域中存在相似的底物结合位点。
Proc Natl Acad Sci U S A. 1999 Jul 20;96(15):8318-20. doi: 10.1073/pnas.96.15.8318.
4
New insights into the ATP-dependent Clp protease: Escherichia coli and beyond.对ATP依赖型Clp蛋白酶的新见解：从大肠杆菌到其他生物
Mol Microbiol. 1999 May;32(3):449-58. doi: 10.1046/j.1365-2958.1999.01357.x.
5
Structure, assembly and regulation of expression of capsules in Escherichia coli.大肠杆菌中荚膜的结构、组装及表达调控
Mol Microbiol. 1999 Mar;31(5):1307-19. doi: 10.1046/j.1365-2958.1999.01276.x.
6
rexB of bacteriophage lambda is an anti-cell death gene.噬菌体λ的rexB是一种抗细胞死亡基因。
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15481-6. doi: 10.1073/pnas.95.26.15481.
7
Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation of a stable outer membrane.waaY、waaQ和waaP在大肠杆菌脂多糖修饰中的作用及其在稳定外膜形成中的作用
J Biol Chem. 1998 Oct 9;273(41):26310-6. doi: 10.1074/jbc.273.41.26310.
8
The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system.ClpXP蛋白酶和ClpAP蛋白酶可降解由SsrA标记系统添加了羧基末端肽尾的蛋白质。
Genes Dev. 1998 May 1;12(9):1338-47. doi: 10.1101/gad.12.9.1338.
9
Regulatory subunits of energy-dependent proteases.能量依赖性蛋白酶的调节亚基。
Cell. 1997 Nov 14;91(4):435-8. doi: 10.1016/s0092-8674(00)80428-6.
10
Proteases and their targets in Escherichia coli.大肠杆菌中的蛋白酶及其作用靶点。
Annu Rev Genet. 1996;30:465-506. doi: 10.1146/annurev.genet.30.1.465.
Zotero 插件