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通过物理化学和免疫学标准无法区分鸡的肌肉β-肌动蛋白和血清白蛋白。

Muscle beta-actinin and serum albumin of the chicken are indistinguishable by physicochemical and immunological criteria.

作者信息

Heizman C W, Müller G, Jenny E, Wilson K J, Landon F, Olomucki A

出版信息

Proc Natl Acad Sci U S A. 1981 Jan;78(1):74-7. doi: 10.1073/pnas.78.1.74.

DOI:10.1073/pnas.78.1.74
PMID:6787597
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318992/
Abstract

Chicken muscle beta-actinin is considered to be one of the "true" myofibrillar components due to its specific binding to isolated myofibrils. Surprisingly, the direct comparison of this muscle protein with serum albumin, both isolated from chicken, showed that they behaved identically under several electrophoretic conditions. Furthermore, immunoreplica gels and double-immunodiffusion tests with antibodies prepared against beta-actinin established the serological identity of both proteins. No significant differences were found by circular dichroic spectroscopy or in amino acid composition. In addition, the amino-terminal sequences of both proteins were identical (H2N-Asp-Ala-Glu-His-Lys-Ser-Glu-Ile-Ala-His-Arg-Tyr-Asn-Asp-Leu-). Combined, these results strongly indicate that muscle beta-actinin and serum albumin are similar, if not identical.

摘要

由于鸡肌肉β - 肌动蛋白能与分离出的肌原纤维特异性结合，所以它被认为是“真正的”肌原纤维成分之一。令人惊讶的是，将这种肌肉蛋白与同样从鸡中分离出的血清白蛋白进行直接比较时发现，在几种电泳条件下它们的行为表现相同。此外，用针对β - 肌动蛋白制备的抗体进行免疫印迹凝胶和双向免疫扩散试验，确定了这两种蛋白质的血清学同一性。通过圆二色光谱法或氨基酸组成分析未发现显著差异。另外，这两种蛋白质的氨基末端序列相同（H2N - Asp - Ala - Glu - His - Lys - Ser - Glu - Ile - Ala - His - Arg - Tyr - Asn - Asp - Leu -）。综合这些结果有力地表明，肌肉β - 肌动蛋白和血清白蛋白即便不完全相同，也是相似的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/cdeefaf41786/pnas00652-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/ccaa9cba8f17/pnas00652-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/de42afa18cfc/pnas00652-0098-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/fab10dcfc15b/pnas00652-0098-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/cdeefaf41786/pnas00652-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/ccaa9cba8f17/pnas00652-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/de42afa18cfc/pnas00652-0098-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/fab10dcfc15b/pnas00652-0098-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9fe3/318992/cdeefaf41786/pnas00652-0099-a.jpg

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Muscle beta-actinin and serum albumin of the chicken are indistinguishable by physicochemical and immunological criteria.通过物理化学和免疫学标准无法区分鸡的肌肉β-肌动蛋白和血清白蛋白。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):74-7. doi: 10.1073/pnas.78.1.74.
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本文引用的文献

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A NEW PROTEIN FACTOR PROMOTING CONTRACTION OF ACTOMYOSIN.一种促进肌动球蛋白收缩的新蛋白质因子。
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Circular dichroism of biological macromolecules.生物大分子的圆二色性
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Studies on purified -actinin. II. Electron microscopic studies on the competitive binding of -actinin and tropomyosin to Z-line extracted myofibrils.肌动蛋白结合蛋白的纯化研究。II. 肌动蛋白结合蛋白与原肌球蛋白对Z线提取肌原纤维竞争性结合的电子显微镜研究
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