Beychok S
Science. 1966 Dec 9;154(3754):1288-99. doi: 10.1126/science.154.3754.1288.
Circular dichroism, the unequal absorption of right and left circularly polarized light, is a manifestation of optical activity in the vicinity of absorption bands. To the experimental scientist interested in the conformation of macromolecules and in the sensitive response of optical activity to conformational alteration, it offers a relatively new and powerful means of understanding the environment of chromophoric residues. As a tool in the elucidation of electronic spectra, it should be useful to the theoretical scientist in identifying weakly allowed absorption bands as well as in providing rotational parameters which can be compared with the developing theory of optical activity. I have stressed application of circular dichroism, to experimental aspects of protein and nucleic acid conformation in solution. Much is still uncertain in particular quantitative details. However, even these early results shed new light and yield new information on the conformation of these molecules.
圆二色性，即对右旋和左旋圆偏振光的不等吸收，是吸收带附近光学活性的一种表现。对于对大分子构象以及光学活性对构象变化的敏感响应感兴趣的实验科学家来说，它提供了一种相对新颖且强大的手段来理解发色团残基的环境。作为阐明电子光谱的一种工具，它对于理论科学家识别弱允许吸收带以及提供可与光学活性发展理论进行比较的旋转参数应该是有用的。我着重介绍了圆二色性在溶液中蛋白质和核酸构象实验方面的应用。特别是在具体的定量细节方面仍有很多不确定之处。然而，即使是这些早期结果也为这些分子的构象带来了新的启示并产生了新的信息。
