Lamberts S W, Visser T J
Eur J Pharmacol. 1981 May 8;71(2-3):337-41. doi: 10.1016/0014-2999(81)90037-6.
The effect of four different preparations of histidyl-proline-diketopiperazine (DKP) on prolactin (PRL) release by rat hemipituitary glands and cultured dispersed normal pituitary cells was studies. Three DKP preparations )0.1-1 micrometer) were ineffective on PRL release in vitro. By combining the results from 12 incubation vials in 3 experiments 1 micrometer of the fourth DKP preparation was shown to suppress PRL release slightly but significantly by 18%, (P less than 0.05). DKP did not enhance the dopamine-mediated inhibition of PRL release and it also did not reverse the TRH-mediated stimulation of PRL release by the pituitary gland in vitro. It was concluded that DKP (in micromolar concentrations) has a very weak PRL release-inhibiting action and is probably not a physiologically acting prolatin-inhibiting factor.
研究了四种不同制剂的组氨酰 - 脯氨酸 - 二酮哌嗪（DKP）对大鼠半垂体和培养的分散正常垂体细胞催乳素（PRL）释放的影响。三种DKP制剂（0.1 - 1微米）在体外对PRL释放无效。通过合并3个实验中12个孵育瓶的结果，发现第四种DKP制剂1微米可轻微但显著地抑制PRL释放18%，（P < 0.05）。DKP并未增强多巴胺介导的PRL释放抑制作用，并且在体外也未逆转促甲状腺激素释放激素（TRH）介导的垂体对PRL释放的刺激作用。得出的结论是，DKP（微摩尔浓度）具有非常弱的PRL释放抑制作用，可能不是一种具有生理作用的催乳素抑制因子。
