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相似文献

1
Effect of tau on the vinblastine-induced aggregation of tubulin.微管相关蛋白tau对长春花碱诱导的微管蛋白聚集的影响。
J Cell Biol. 1981 Jun;89(3):680-3. doi: 10.1083/jcb.89.3.680.
2
Contrasting roles of tau and microtubule-associated protein 2 in the vinblastine-induced aggregation of brain tubulin.微管相关蛋白tau和微管相关蛋白2在长春碱诱导的脑微管蛋白聚集中的相反作用。
J Biol Chem. 1984 Oct 25;259(20):12890-8.
3
Temperature sensitivity of vinblastine-induced tubulin polymerization in the presence of microtubule-associated proteins.在存在微管相关蛋白的情况下长春碱诱导微管蛋白聚合的温度敏感性
J Protein Chem. 1992 Oct;11(5):509-15. doi: 10.1007/BF01025028.
4
Effects of Tau and MAP2 on the interaction of maytansine with tubulin: inhibitory effect of maytansine on vinblastine-induced aggregation of tubulin.Tau和MAP2对美登素与微管蛋白相互作用的影响：美登素对长春碱诱导的微管蛋白聚集的抑制作用
Cancer Res. 1985 Oct;45(10):5004-10.
5
Vinblastine-induced aggregation of brine shrimp (Artemia) tubulin.长春碱诱导卤虫微管蛋白聚集。
Biochim Biophys Acta. 1986 Jul 16;882(3):419-26. doi: 10.1016/0304-4165(86)90266-7.
6
The removal of the carboxy-terminal region of tubulin favors its vinblastine-induced aggregation into spiral-like structures.去除微管蛋白的羧基末端区域有利于其在长春碱诱导下聚集成螺旋状结构。
Arch Biochem Biophys. 1986 Sep;249(2):611-5. doi: 10.1016/0003-9861(86)90040-8.
7
Removal of the projection domain of microtubule-associated protein 2 alters its interaction with tubulin.去除微管相关蛋白2的投射结构域会改变其与微管蛋白的相互作用。
J Protein Chem. 1994 May;13(4):381-91. doi: 10.1007/BF01901694.
8
Different effects of vinblastine on the polymerization of isotypically purified tubulins from bovine brain.长春碱对从牛脑中等型纯化微管蛋白聚合的不同影响。
Invest New Drugs. 2003 Feb;21(1):3-13. doi: 10.1023/a:1022946305242.
9
Biochemical effects of Navelbine on tubulin and associated proteins.诺维本对微管蛋白及相关蛋白的生化作用。
Semin Oncol. 1989 Apr;16(2 Suppl 4):9-14.
10
Effect of estramustine phosphate on the assembly of trypsin-treated microtubules and microtubules reconstituted from purified tubulin with either tau, MAP2, or the tubulin-binding fragment of MAP2.磷酸雌莫司汀对经胰蛋白酶处理的微管以及由纯化微管蛋白与tau、微管相关蛋白2（MAP2）或MAP2的微管结合片段重构的微管组装的影响。
Arch Biochem Biophys. 1987 Aug 15;257(1):123-30. doi: 10.1016/0003-9861(87)90550-9.

引用本文的文献

1
Removal of the projection domain of microtubule-associated protein 2 alters its interaction with tubulin.去除微管相关蛋白2的投射结构域会改变其与微管蛋白的相互作用。
J Protein Chem. 1994 May;13(4):381-91. doi: 10.1007/BF01901694.
2
Evidence for three "classes" of microtubules in the interpolar space of the mitotic micronucleus of a ciliate and the participation of the nuclear envelope in conferring stability to microtubules.在纤毛虫有丝分裂微核的极间空间中存在三类微管的证据以及核膜在赋予微管稳定性方面的作用。
Chromosoma. 1982;85(5):687-706. doi: 10.1007/BF00330781.
3
A microtubule-associated protein antigen unique to mitotic spindle microtubules in PtK1 cells.一种PtK1细胞有丝分裂纺锤体微管特有的微管相关蛋白抗原。
J Cell Biol. 1983 Feb;96(2):424-34. doi: 10.1083/jcb.96.2.424.
4
Axonal transport of a subclass of tau proteins: evidence for the regional differentiation of microtubules in neurons.tau蛋白亚类的轴突运输：神经元中微管区域分化的证据。
Proc Natl Acad Sci U S A. 1984 Mar;81(5):1570-4. doi: 10.1073/pnas.81.5.1570.
5
Immunoelectron microscopic localization of the 210,000-mol wt microtubule-associated protein in cultured cells of primates.210,000道尔顿微管相关蛋白在灵长类动物培养细胞中的免疫电子显微镜定位
J Cell Biol. 1981 Nov;91(2 Pt 1):438-45. doi: 10.1083/jcb.91.2.438.
6
Comparison of the effects of microtubule-associated protein 2 and tau on the packing density of in vitro assembled microtubules.微管相关蛋白2和tau对体外组装微管堆积密度影响的比较。
Proc Natl Acad Sci U S A. 1987 Nov;84(21):7783-7. doi: 10.1073/pnas.84.21.7783.
7
Differential in vitro action of S-12363, a new vinblastine derivative, and of its epimer on microtubule proteins.新型长春碱衍生物S-12363及其差向异构体对微管蛋白的体外差异作用
Cancer Chemother Pharmacol. 1991;28(6):434-40. doi: 10.1007/BF00685819.

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.还原和S-羧甲基化蛋白质的制备及酶促水解
J Biol Chem. 1963 Feb;238:622-7.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Structure of microtubular crystals induced by vinblastine in vitro.长春碱在体外诱导形成的微管晶体的结构
J Cell Biol. 1970 Jan;44(1):234-8. doi: 10.1083/jcb.44.1.234.
5
Vinblastine and microtubules. I. Induction and isolation of crystals from sea urchin oocytes.
Exp Cell Res. 1971 May;66(1):129-36. doi: 10.1016/s0014-4827(71)80020-4.
6
Microtubule-macrotubule transitions: intermediates after exposure to the mitotic inhibitor vinblastine.微管-巨管转变：暴露于有丝分裂抑制剂长春花碱后的中间体。
Science. 1974 Dec 27;186(4170):1219-21. doi: 10.1126/science.186.4170.1219.
7
A protein factor essential for microtubule assembly.微管组装所必需的一种蛋白质因子。
Proc Natl Acad Sci U S A. 1975 May;72(5):1858-62. doi: 10.1073/pnas.72.5.1858.
8
Negatively stained vinblastine aggregates.负染的长春花碱聚集体。
Ann N Y Acad Sci. 1975 Jun 30;253:51-2. doi: 10.1111/j.1749-6632.1975.tb19191.x.
9
Microtubule assembly in vitro. Purification of assembly-promoting factors.体外微管组装。组装促进因子的纯化。
Eur J Biochem. 1977 Aug 15;78(1):167-74. doi: 10.1111/j.1432-1033.1977.tb11726.x.
10
Differences in surface morphology of microtubules reconstituted from pure brain tubulin using two different microtubule-associated proteins: the high molecular weight MAP 2 proteins and tau proteins.使用两种不同的微管相关蛋白（高分子量微管相关蛋白2和tau蛋白）由纯脑微管蛋白重构的微管在表面形态上的差异。
Eur J Cell Biol. 1979 Jun;19(2):175-83.

微管相关蛋白tau对长春花碱诱导的微管蛋白聚集的影响。

Effect of tau on the vinblastine-induced aggregation of tubulin.

作者信息

Ludueña R F, Fellous A, Francon J, Nunez J, McManus L

出版信息

J Cell Biol. 1981 Jun;89(3):680-3. doi: 10.1083/jcb.89.3.680.

DOI:10.1083/jcb.89.3.680
PMID:6788779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2111808/
Abstract

Two microtubule-associated proteins, tau and the high molecular weight microtubule-associated protein 2 (MAP 2), were purified from rat brain microtubules. Addition of either protein to pure tubulin caused microtubule assembly. In the presence of tau and 10 microM vinblastine, tubulin aggregated into spiral structures. If tau was absent, or replaced by MAP 2, little aggregation occurred in the presence of vinblastine. Thus, vinblastine may be a useful probe in elucidating the individual roles of tau and MAP 2 in microtubule assembly.

摘要

从大鼠脑微管中纯化出两种微管相关蛋白，即tau蛋白和高分子量微管相关蛋白2（MAP 2）。将这两种蛋白中的任何一种添加到纯微管蛋白中都会导致微管组装。在tau蛋白和10微摩尔长春花碱存在的情况下，微管蛋白聚集成螺旋结构。如果没有tau蛋白，或者用MAP 2替代，在长春花碱存在的情况下几乎不会发生聚集。因此，长春花碱可能是阐明tau蛋白和MAP 2在微管组装中各自作用的有用探针。