Rostas J A, Leung W N, Jeffrey P L
Neurosci Lett. 1981 Jul 2;24(2):155-60. doi: 10.1016/0304-3940(81)90240-8.
Isolated synaptic junction fractions from adult chicken brain were found to contain a number of glycoprotein glycosyltransferase with different donor and acceptor specificities. The activities of sialyl- and galactosyltransferases towards endogenous acceptors in the synaptic junction fraction were low but increased greatly after the addition of deglycosylated fetuin or mucin as exogenous acceptors. The activity of the fucosyltransferases towards endogenous acceptors in the fraction was much higher than that of the other transferases. The addition of deglycosylated fetuin caused a smaller increase in the activity than with the other transferases and deglycosylated mucin had no effect. We find no evidence for the enrichment of any glycosyltransferase in the synaptic junction. If anything, our results suggest that the synaptic junction may be specifically depleted in one type of fucosyltransferase.
研究发现，从成年鸡脑中分离出的突触连接部分含有多种具有不同供体和受体特异性的糖蛋白糖基转移酶。突触连接部分中唾液酸转移酶和半乳糖基转移酶对内源性受体的活性较低，但在添加去糖基化的胎球蛋白或粘蛋白作为外源性受体后，活性大幅增加。该部分中岩藻糖基转移酶对内源性受体的活性远高于其他转移酶。添加去糖基化的胎球蛋白后，其活性增加幅度小于其他转移酶，而去糖基化的粘蛋白则没有影响。我们没有发现任何糖基转移酶在突触连接中富集的证据。相反，我们的结果表明，突触连接可能在一种岩藻糖基转移酶中特异性减少。
