Noll F, Lutsch G, Bommer U A, Theise H, Bielka H
Acta Histochem Suppl. 1980;22:299-308.
Due to their high specificity antibodies reveal possibilities for a detailed analysis of structure and function of eukaryotic ribosomes. By means of specific antibodies against single ribosomal proteins we could (1) localize immunoelectron microscopically certain proteins at the surface of ribosomes and (2) determine their role within protein biosynthesis by the binding of the initiation factor eIF-2 to the small ribosomal subunit. The combination of the results of both methods provides evidence of the localization of the peptidyl-tRNA binding site in the head area of the small subunit of ribosomes.
由于其高度特异性，抗体为详细分析真核核糖体的结构和功能提供了可能。借助针对单个核糖体蛋白的特异性抗体，我们能够（1）通过免疫电子显微镜将某些蛋白质定位在核糖体表面，以及（2）通过起始因子eIF-2与小核糖体亚基的结合来确定它们在蛋白质生物合成中的作用。两种方法结果的结合为肽基-tRNA结合位点在核糖体小亚基头部区域的定位提供了证据。
