Victoria E J, Mahan L C
Biochim Biophys Acta. 1981 Jun 22;644(2):226-32. doi: 10.1016/0005-2736(81)90379-5.
The properties of integral membrane proteins obtained by dilute alkali extraction of red cell ghosts were examined. A variety of conditions promoted the disulfide-mediated aggregation of integral membrane proteins, principally band 3. Procedural modifications which minimized aggregation were the use of EDTA and S-alkylation. Integral membrane proteins were solubilized under non-denaturing conditions using Brij 36T, a lauryl polyoxyethylene ether with an NMR-determined average chain length of 8.2 (oxyethylene) units. Detergent gel filtration revealed a chromatographic shoulder due to aggregated band 3 when membrane proteins were not alkylated. Analyses of the column profile also revealed a discrete peak for sialoglycoproteins and two phosphate peaks, an early one due to phospholipid and a later one not identified, but probably due to phosphoinositide.
对通过稀碱提取红细胞血影获得的整合膜蛋白的性质进行了研究。多种条件促进了整合膜蛋白（主要是带3蛋白）的二硫键介导的聚集。使聚集最小化的程序修改是使用乙二胺四乙酸（EDTA）和S-烷基化。使用Brij 36T在非变性条件下溶解整合膜蛋白，Brij 36T是一种月桂基聚氧乙烯醚，核磁共振测定的平均链长为8.2个（氧化乙烯）单元。当膜蛋白未被烷基化时，去污剂凝胶过滤显示由于带3蛋白聚集而出现色谱肩峰。对柱谱的分析还显示了唾液糖蛋白的一个离散峰和两个磷酸盐峰，较早的一个归因于磷脂，较晚的一个未鉴定，但可能归因于磷酸肌醇。
