生长和非生长状态的梨形四膜虫中儿茶酚胺的高效液相色谱分析

High performance liquid chromatographic analysis of catecholamines in growing and non-growing Tetrahymena pyriformis.

作者信息

Goldman M E, Gundersen R E, Erickson C K, Thompson G A

出版信息

Biochim Biophys Acta. 1981 Aug 17;676(2):221-5. doi: 10.1016/0304-4165(81)90190-2.

Abstract

Tetrahymena pyriformis, strains NT-1 and W, harvested in logarithmic (growing) and stationary (non-growing) phases, were found by high-performance liquid chromatography to contain considerable quantities of dopamine. In addition, small amounts of epinephrine and norepinephrine were detected. Logarithmic-phase strain NT-1 cells contained 249 +/- 44 pg dopamine/10(6) cells compared to 477 +/- 42 pg/10(6) cells for logarithmic-phase strain W cells for logarithmic-phase strain W cells. The dopamine content of stationary-phase cells was approximately half the value of the logarithmic-phase cells. There was a significant amount of dopamine in the growth medium from stationary-phase cultures and, to a lesser extent, logarithmic-phase cells.

摘要

梨形四膜虫NT-1株和W株，分别处于对数生长期（生长阶段）和稳定期（非生长阶段）收获，通过高效液相色谱法发现含有大量多巴胺。此外，还检测到少量肾上腺素和去甲肾上腺素。对数生长期的NT-1株细胞含有249±44 pg多巴胺/10⁶个细胞，而对数生长期的W株细胞为477±42 pg/10⁶个细胞。稳定期细胞的多巴胺含量约为对数生长期细胞的一半。在稳定期培养物的生长培养基中存在大量多巴胺，对数生长期细胞中含量较少。