Nielsen K, Stilwell K, Stemshorn B, Duncan R
J Clin Microbiol. 1981 Jul;14(1):32-8. doi: 10.1128/jcm.14.1.32-38.1981.
It was demonstrated by a radioimmunoassay procedure that Brucella abortus agglutinins from noninfected cattle sera, absorbed to B. abortus antigen and eluted with ethylenediaminetetraacetic acid (EDTA), was immunoglobulin M that bound to that bacterium by its Fc portion. The EDTA-eluted immunoglobulin M agglutinated intact B. abortus cells but not erythrocytes treated with B. abortus lipopolysaccharide. The specificity of the EDTA-eluted immunoglobulin was for B. abortus, although a small titer to Yersinia enterocolitica serotype O:9 was observed. In contrast, immunoglobulin M purified from the serum of a cow injected 7 days previously with heat-killed B. abortus bound to the antigen by its Fab portion, was not labile to EDTA treatment, cross-reacted extensively with Y. enterocolitica serotype O:9, and agglutinated various other bacterial antigens and normal erythrocytes.
通过放射免疫测定法证明，来自未感染牛血清的布鲁氏菌流产凝集素，吸附到流产布鲁氏菌抗原上并用乙二胺四乙酸（EDTA）洗脱后，是通过其Fc部分与该细菌结合的免疫球蛋白M。经EDTA洗脱的免疫球蛋白M能凝集完整的流产布鲁氏菌细胞，但不能凝集经流产布鲁氏菌脂多糖处理的红细胞。经EDTA洗脱的免疫球蛋白的特异性针对流产布鲁氏菌，尽管观察到对小肠结肠炎耶尔森菌血清型O:9有少量效价。相比之下，从7天前注射热灭活流产布鲁氏菌的奶牛血清中纯化的免疫球蛋白M通过其Fab部分与抗原结合，对EDTA处理不敏感，与小肠结肠炎耶尔森菌血清型O:9广泛交叉反应，并能凝集各种其他细菌抗原和正常红细胞。
