Prokůpek K, Dvorák R, Polácek R
Vet Med (Praha). 1981 May;26(5):279-90.
In experimental immunization of sheep and goats against anthrax the vaccine containing our own protective antigen (PA) whose serological activity was evaluated by the titre 1:16 was used. PA was adsorbed on aluminium hydroxide gel at the ratio 30 parts of gel and 100 parts of antigen. In the control experiment the doses of the virulent anthrax strain were as follows: 10(6) (sheep) and 5.10(6) (goats). The single vaccination provided only 60% protection, the repeated 100% protection. By the titration of antibodies of the vaccinated animals was proved that the peak titres (1:80 to 1:160) were reached two months after vaccination. Then the titre decreased gradually, and in approximately two months' time it returned to the initial value. As apparent from these experimental results, a reliable protection by PA vaccine can be achieved only by double immunization, best at 14-day intervals.
在绵羊和山羊的炭疽实验性免疫中，使用了含有我们自己的保护性抗原（PA）的疫苗，其血清学活性通过1:16的滴度进行评估。PA以30份凝胶与100份抗原的比例吸附在氢氧化铝凝胶上。在对照实验中，强毒炭疽菌株的剂量如下：10(6)（绵羊）和5×10(6)（山羊）。单次接种仅提供60%的保护，重复接种则提供100%的保护。通过对接种动物抗体的滴定证明，接种后两个月达到峰值滴度（1:80至1:160）。然后滴度逐渐下降，大约两个月后恢复到初始值。从这些实验结果可以看出，只有通过两次免疫，最好间隔14天，才能通过PA疫苗实现可靠的保护。
