Yamaguchi Y, Nagai R
J Cell Sci. 1981 Apr;48:193-205. doi: 10.1242/jcs.48.1.193.
The organization of the microfilaments in epidermal cells of Vallisneria leaves was investigated with respect to the induction of cytoplasmic streaming (secondary streaming). In many of the epidermal cells, cytoplasm exhibited rotational streaming along the anticlinal wall of the cell after exposure around the anticlinal wall. The bundles were arrayed in parallel to the streaming direction. They were recognized usually as 10-40 closely packed dense dots in cross-section. The spacing between bundles was not even. Bundles tended to form groups of 4 to 5 in which the spacing between bundles was usually 0.3 to 0.5 micrometer. The microfilaments were identified as F-actin. Together with the fact that rotational streaming in Vallisneria cells by cytochalasin B, the motile mechanism of secondary streaming was concluded to be similar in its essential features to the cytoplasmic streaming seen in Characean cell (primary streaming). In epidermal cells that had been kept under low-intensity light the cytoplasm and the cytoplasmic streaming occurred in these cells. The bundles of microfilaments remained in the very thin layer of cytoplasm lining the anticlinal wall, although they were fewer and somewhat loosely packed. EGTA at appropriate concentration could induce cytoplasmic streaming in these cells. The mechanism of the induction is discussed on the basis of the effectiveness of EGTA and the requirement of a low concentration of free Ca2+ for cytoplasmic streaming in Characean cells.
针对细胞质流动（次生流动）的诱导，研究了苦草叶片表皮细胞中微丝的组织情况。在许多表皮细胞中，细胞质在围绕垂周壁暴露后，沿着细胞的垂周壁呈现出旋转流动。微丝束与流动方向平行排列。在横切面上，它们通常被识别为10 - 40个紧密排列的致密点。微丝束之间的间距不均匀。微丝束倾向于形成4到5个一组，其中微丝束之间的间距通常为0.3到0.5微米。微丝被鉴定为F - 肌动蛋白。再加上用细胞松弛素B处理苦草细胞会导致旋转流动这一事实，得出次生流动的运动机制在其基本特征上与轮藻细胞中所见的细胞质流动（原生流动）相似的结论。在置于低强度光照下的表皮细胞中，这些细胞中出现了细胞质和细胞质流动。微丝束保留在垂周壁内衬的非常薄的细胞质层中，尽管它们数量较少且排列有些松散。适当浓度的乙二醇双乙醚二胺四乙酸（EGTA）可以诱导这些细胞中的细胞质流动。基于EGTA的有效性以及轮藻细胞中细胞质流动对低浓度游离Ca2+的需求，对诱导机制进行了讨论。
