Wolfe H G, Bartke A, Amador A, Van Sickle M, Dalterio S, Brown D
J Endocrinol. 1981 Sep;90(3):367-73. doi: 10.1677/joe.0.0900367.
Mice were selected on the basis of ovulatory responses to injections of pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG). Various parameters of pituitary and gonadal function as well as responsiveness to exogenous gonadotrophins were examined in males from high induced-ovulation rate (HIOV) and low induced-ovulation rate (LIOV) lines. Testicular weight, seminal vesicle weight and plasma LH levels were lower in HIOV than in LIOV males, while plasma concentrations of FSH and testosterone did not differ. Binding of FSH, but not of LH, in the testes was significantly higher in HIOV mice. Twenty-four hours after administration of hCG, plasma testosterone levels were higher and testicular LH binding sites appeared more depleted in HIOV than in LIOV males. Production of testosterone by decapsulated testes in vitro was significantly higher in HIOV than in LIOV mice under basal conditions, as well as in the presence of LH, FSH, hCG or PMSG. It was concluded that selection for differences in gonadotrophin-induced ovulation rate produced correlated differences in the steroidogenic response of the testes to gonadotrophins and that these differences may be due to divergence in the number of gonadal FSH binding sites.
根据对注射孕马血清促性腺激素（PMSG）和人绒毛膜促性腺激素（hCG）的排卵反应来选择小鼠。在高诱导排卵率（HIOV）和低诱导排卵率（LIOV）品系的雄性小鼠中，检测了垂体和性腺功能的各种参数以及对外源性促性腺激素的反应性。HIOV雄性小鼠的睾丸重量、精囊重量和血浆LH水平低于LIOV雄性小鼠，而血浆FSH和睾酮浓度没有差异。HIOV小鼠睾丸中FSH的结合显著高于LH的结合。注射hCG 24小时后，HIOV雄性小鼠的血浆睾酮水平更高，睾丸LH结合位点的消耗比LIOV雄性小鼠更多。在基础条件下以及存在LH、FSH、hCG或PMSG的情况下，HIOV小鼠去包膜睾丸体外睾酮生成显著高于LIOV小鼠。得出的结论是，对促性腺激素诱导排卵率差异的选择导致睾丸对促性腺激素的类固醇生成反应产生相关差异，并且这些差异可能是由于性腺FSH结合位点数量的差异所致。
