眼虫细胞质苯丙氨酸转移核糖核酸的核苷酸序列。关于眼虫可能应归类于动物界而非植物界的证据。 - Suppr | 超能文献

眼虫细胞质苯丙氨酸转移核糖核酸的核苷酸序列。关于眼虫可能应归类于动物界而非植物界的证据。

The nucleotide sequence of Euglena cytoplasmic phenylalanine transfer RNA. Evidence for possible classifications of Euglena among the animal rather than the plant kingdom.

作者信息

Chang S H, Hecker L I, Brum C K, Schnabel J J, Heckman J E, Silberklang M, RajBhandary U L, Barnett W E

出版信息

Nucleic Acids Res. 1981 Jul 10;9(13):3199-204. doi: 10.1093/nar/9.13.3199.

DOI:10.1093/nar/9.13.3199

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327342/

Abstract

The nucleotide sequence of cytoplasmic phenylalanine tRNA from Euglena gracilis has been elucidated using procedures described previously for the corresponding chloroplastic tRNA [Cell, 9, 717 (1976)]. The sequence is: pG-C-C-G-A-C-U-U-A-m(2)G-C-U-Cm-A-G-D-D-G-G-G-A-G-A-G-C-m(2)2G-psi-psi-A-G-A-Cm -U-Gm-A-A-Y-A-psi-C-U-A-A-A-G-m(7)G-U-C-*C-C-U-G-G-T-psi-C-G-m(1)A-U-C-C-C-G-G- G-A-G-psi-C-G-G-C-A-C-C-A. Like other tRNA Phes thus far sequenced, this tRNA has a chain length of 76 nucleotides. The sequence of E. gracilis cytoplasmic tRNA Phe is quite different (27 nucleotides out of 76 different) from that of the corresponding chloroplastic tRNA but is surprisingly similar (72 out of 76 nucleotides identical) to that of tRNA Phe from mammalian cytoplasm. This extent of sequence homology even exceeds that found between E. gracilis and wheat germ cytoplasmic tRNA Phe. These findings raise interesting questions on the evolution of tRNAs and the taxonomy of Euglena.

摘要

利用先前描述的针对相应叶绿体tRNA的方法[《细胞》，9，717（1976）]，已阐明了纤细裸藻细胞质苯丙氨酸tRNA的核苷酸序列。序列如下：pG-C-C-G-A-C-U-U-A-m(2)G-C-U-Cm-A-G-D-D-G-G-G-A-G-A-G-C-m(2)2G-ψ-ψ-A-G-A-Cm-U-Gm-A-A-Y-A-ψ-C-U-A-A-A-G-m(7)G-U-C-*C-C-U-G-G-T-ψ-C-G-m(1)A-U-C-C-C-G-G-G-A-G-ψ-C-G-G-C-A-C-C-A。与迄今已测序的其他苯丙氨酸tRNA一样，这种tRNA的链长为76个核苷酸。纤细裸藻细胞质苯丙氨酸tRNA的序列与相应叶绿体tRNA的序列有很大不同（76个中有27个不同），但令人惊讶的是，它与哺乳动物细胞质苯丙氨酸tRNA的序列非常相似（76个中有72个相同）。这种序列同源程度甚至超过了纤细裸藻与小麦胚细胞质苯丙氨酸tRNA之间的同源程度。这些发现引发了关于tRNA进化和裸藻分类学的有趣问题。