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醛类对多胺代谢的影响。I. 用于测定酶促反应中“体外”产生的二氧化碳的方法:其在评估S-腺苷甲硫氨酸脱羧酶(SAMD)活性中的应用。

Effect of aldehydes on polyamine metabolism. I. Method used to determine CO2 produced "in vitro" in enzymatic reactions: its application in evaluation of S-adenosylmethionine decarboxylase (SAMD) activity.

作者信息

Chiarpotto E, Albano E, Paradisi L, Poli G, Gravela E

出版信息

Boll Soc Ital Biol Sper. 1981 Jun 15;57(11):1195-201.

PMID:6793045
Abstract

In this paper the Authors describe a new method they have adopted (in studies reported in accompanying papers II and III) for measuring S-adenosylmethionine decarboxylase activity in entire cells and cell fractions. The new method is a general one for recovering and measuring labelled carbon dioxide formed "in vitro" during enzymatic reactions. It presents several advantages when compared to traditional methods.

摘要

在本文中,作者描述了一种他们采用的新方法(在随附论文II和III中报道的研究中),用于测量全细胞和细胞组分中的S-腺苷甲硫氨酸脱羧酶活性。这种新方法是一种用于回收和测量酶促反应过程中“体外”形成的标记二氧化碳的通用方法。与传统方法相比,它具有几个优点。

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