White F H, Gohari K
Br J Cancer. 1981 Sep;44(3):440-50. doi: 10.1038/bjc.1981.203.
The present study was designed to establish whether there are changes in hemidesmosomal distribution during defined stages of chemical carcinogenesis in hamster cheek-pouch epithelium. 0.5% DMBA in liquid paraffin was applied thrice weekly to hamster pouches and tissue samples were obtained at regular intervals and assigned to hyperplastic, dysplastic and carcinomatous groups on the basis of histological criteria. Untreated pouches served as controls. Following a strict sampling regime, electron micrographs were obtained from the epithelial-connective tissue junction and, using stereological intersection counting, the relative surface area of basal plasma membrane (BPM) occupied by hemidesmosomes was estimated. In normal epithelium 40% of the BPM is occupied by hemidesmosomes. During carcinogenesis, values decrease progressively and significantly to 35% in hyperplasia, 28% in dysplasia and 13% in carcinoma. A decrease in the relative area of hemidesmosomes would therefore appear to contribute to the increased motility of epithelial cells during a connective-tissue invasion and cellular metastasis.
本研究旨在确定在仓鼠颊囊上皮化学致癌作用的特定阶段，半桥粒分布是否存在变化。将液体石蜡中的0.5%二甲基苯并蒽每周三次涂抹于仓鼠颊囊，定期获取组织样本，并根据组织学标准将其分为增生、发育异常和癌三组。未处理的颊囊作为对照。按照严格的采样方案，从上皮-结缔组织连接处获取电子显微照片，并使用体视学交叉计数法，估计半桥粒占据的基底质膜（BPM）的相对表面积。在正常上皮中，40%的BPM被半桥粒占据。在致癌过程中，该值逐渐显著下降，增生时降至35%，发育异常时降至28%，癌时降至13%。因此，半桥粒相对面积的减少似乎有助于上皮细胞在结缔组织侵袭和细胞转移过程中运动性的增加。
