White F H, Gohari K, Smith C J
Diagn Histopathol. 1981 Oct-Dec;4(4):307-33.
The epithelial changes observed during hamster cheek pouch-DMBA carcinogenesis were classified as hyperplasia, dysplasia and carcinoma stages; carcinomas were usually well differentiated. During carcinogenesis, epithelial thickness increased and in dysplasia and carcinoma stages superficial cells exhibited a marked shift from ortho- to parakeratinization. Marked changes in intercellular organelles were observed and intracellular spaces increased progressively in size. Desmosomes were less commonly seen during carcinogenesis whereas the frequency of gap junctions appeared to increase. The epithelial-connective tissue junction exhibited prominent changes with increasing irregularity, discontinuity and duplication of the lamina densa and a decreasing frequency of hemidesmosomes. Cytoplasmic processes, some containing lysosomal dense bodies, often passed through breaks in the lamina densa and extended into a relatively structureless connective tissue. The results are discussed in relation to the changes in cellular organelles occurring in abnormal epithelial differentiation mediated by the carcinogen and to possible factors which might be involved in the process of cellular invasion.
在仓鼠颊囊-二甲基苯并蒽致癌过程中观察到的上皮变化被分为增生、发育异常和癌阶段；癌通常分化良好。在致癌过程中，上皮厚度增加，在发育异常和癌阶段，表层细胞表现出从正角化到不全角化的明显转变。观察到细胞间细胞器有明显变化，细胞内间隙大小逐渐增加。在致癌过程中桥粒较少见，而缝隙连接的频率似乎增加。上皮-结缔组织连接表现出明显变化，致密板越来越不规则、不连续和重复，半桥粒频率降低。细胞质突起，有些含有溶酶体致密小体，常常穿过致密板的断裂处并延伸到相对无结构的结缔组织中。结合致癌物介导的异常上皮分化中发生的细胞器变化以及可能参与细胞侵袭过程的因素对结果进行了讨论。
