Turgenbaeva D A, Kaprel'iants A S, Zinov'eva M E, Lukoianova M A, Ostrovskiĩ D N
Biokhimiia. 1981 Apr;46(4):642-51.
Using low (0.0025 -- 0.025%) concentrations of Triton X-100, the correlation between the decrease of NADN- and malate oxidase activities and NADH- and malate dehydrogenase release in large fragments of Micrococcus lysodeikticus membranes was established. This was accompanied by membrane suspension clearance and a decrease of microviscosity of the membrane lipid component. Using NADH-dehydrogenase, it was shown that the attachment of NADH-dehydrogenase to the membrane treated with glutaric aldehyde occurs in two steps, this being indicative of different environment of this enzyme in the membrane. The data obtained are discussed in terms of laterally heterogenous structure of the bacterial membrane with respect to the electron transport enzymes, in particular in favour of an existence of individual sites of the membrane containing dehydrogenases rather than other respiratory chain components.
使用低浓度（0.0025% - 0.025%）的曲拉通X-100，建立了溶壁微球菌膜大片段中NADN和苹果酸氧化酶活性降低与NADH和苹果酸脱氢酶释放之间的相关性。这伴随着膜悬浮液的澄清以及膜脂质成分微粘度的降低。使用NADH脱氢酶表明，NADH脱氢酶与经戊二醛处理的膜的附着分两步进行，这表明该酶在膜中的环境不同。就细菌膜相对于电子传递酶的横向异质结构而言，特别是支持存在含有脱氢酶而不是其他呼吸链成分的膜的单个位点，对所获得的数据进行了讨论。
