Walker I G
Carcinogenesis. 1981;2(8):691-5. doi: 10.1093/carcin/2.8.691.
4-Nitroquinoline-1-oxide (4NQO) damage to DNA and its repair in normal and xeroderma pigmentosum (XP) fibroblast stains were followed by sedimentation in an alkaline sucrose gradient. Two forms of analysis were employed. In the brief lysis technique, the cells were exposed to alkali for 30 min before centrifuging. In the long lysis technique, the cells were exposed to alkali overnight before centrifuging. The fibroblast cultures were derived from 6 normal individuals, 2 XP variants, 2 from complementation group C and 1 each from complementation groups A, B and E. by means of the brief lysis technique all of the fibroblasts were found to repair the 4NQO induced damage. By means of the long lysis technique the fibroblasts from normal individuals, from XP variants and from XP group E were found to repair the 4NQO induced damage. The fibroblasts from groups A, B and C showed no repair of the lesion. This latter result, on the repair capability of 4NQO damaged XP cells, correlates with the repair capability of XP cells shown by other methods. It suggest that if sedimentation analysis in alkaline sucrose is to be used to demonstrate the induction of chemically induced damage to DNA and its repair, both brief and long lysis periods should be employed.
通过碱性蔗糖梯度沉降法，对4-硝基喹啉-1-氧化物（4NQO）对正常及着色性干皮病（XP）成纤维细胞株DNA的损伤及其修复情况进行了跟踪研究。采用了两种分析形式。在短时间裂解技术中，细胞在离心前先暴露于碱中30分钟。在长时间裂解技术中，细胞在离心前先暴露于碱中过夜。成纤维细胞培养物来源于6名正常个体、2名XP变异型个体、2名C互补组个体以及A、B和E互补组各1名个体。通过短时间裂解技术发现，所有成纤维细胞都能修复4NQO诱导的损伤。通过长时间裂解技术发现，正常个体、XP变异型个体以及XP E组的成纤维细胞能修复4NQO诱导的损伤。A、B和C组的成纤维细胞未显示出损伤修复。4NQO损伤的XP细胞的后一修复能力结果，与其他方法显示的XP细胞修复能力相关。这表明，如果要用碱性蔗糖沉降分析法来证明化学诱导的DNA损伤及其修复，应同时采用短时间和长时间裂解期。
