Wiederrecht G J, Paton D R, Brown G M
J Biol Chem. 1981 Oct 25;256(20):10399-402.
A compound that is involved in the biosynthesis of the drosopterin eye pigments has been isolated from the heads of Drosophila melanogaster. Analyses of this compound by chemical, mass spectral, and proton nuclear magnetic resonance techniques in conjunction with biochemical considerations provide evidence for the structure 2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b][1,4]diazepine (PDA). At least three eye pigments (drosopterin, isodrosopterin, and aurodrosopterin) are synthesized when PDA and 2-amino-4-oxo-(D-erythro-1',2',3'-trihydroxypropyl)-7.8-dihydropteridine triphosphate (dihydroneopterin triphosphate) are incubated with Mg2+ and protein fractions prepared from Drosophila heads. The synthesis of aurodrosopterin, in addition, requires reduced pyridine nucleotide. Other evidence suggests that dihydroneopterin triphosphate is a biosynthetic precursor of PDA.
一种参与果蝇蝶呤眼色素生物合成的化合物已从黑腹果蝇的头部中分离出来。通过化学、质谱和质子核磁共振技术对该化合物进行分析，并结合生化方面的考虑，为其结构2-氨基-4-氧代-6-乙酰基-7,8-二氢-3H,9H-嘧啶并[4,5-b][1,4]二氮杂䓬（PDA）提供了证据。当PDA和2-氨基-4-氧代-（D-赤藓糖-1',2',3'-三羟基丙基）-7,8-二氢蝶呤三磷酸（二氢新蝶呤三磷酸）与Mg2+以及从果蝇头部制备的蛋白质组分一起孵育时，至少会合成三种眼色素（果蝇蝶呤、异果蝇蝶呤和金果蝇蝶呤）。此外，金果蝇蝶呤的合成还需要还原型吡啶核苷酸。其他证据表明，二氢新蝶呤三磷酸是PDA的生物合成前体。
