通过用来自日本人的蛋白A分级分离的正常IgG蛋白免疫兔子制备抗Gm血清：进一步研究
Preparation of anti-Gm sera by immunization of rabbits with protein A-fractionated normal IgG proteins from Japanese: further study.
作者信息
Tamaki Y, Kishida T, Shibata K, Takahashi N, Fukuda M
出版信息
J Immunol Methods. 1981;45(2):177-82. doi: 10.1016/0022-1759(81)90211-8.
A method is described for the production of rabbit anti-Gm sera using normal IgG proteins and fragments thereof as the immunogens. Anti-G3m(16) and anti-G3m(21) were readily producible by immunizations with protein A-purified IgG3 proteins of G3m(13, 15, 16) and G3m(21) type, respectively. Anti-G1m(2), anti-G1m(3), and anti-G3m(5) were obtained by immunizations with type Gm(1, 2, 21) Fc fragments, type Gm(1, 3, 5, 13) IgG1-rich IgG, and type G3m(5, 13, 21) IgG3, respectively. These immune sera can distinguish the 9 Gm phenotypes in the Japanese.
描述了一种使用正常IgG蛋白及其片段作为免疫原生产兔抗Gm血清的方法。分别用G3m(13, 15, 16)型和G3m(21)型的蛋白A纯化的IgG3蛋白免疫，可轻易产生抗G3m(16)和抗G3m(21)。分别用Gm(1, 2, 21) Fc片段、富含Gm(1, 3, 5, 13) IgG1的IgG和G3m(5, 13, 21) IgG3免疫，可获得抗G1m(2)、抗G1m(3)和抗G3m(5)。这些免疫血清可区分日本人的9种Gm表型。
Zotero 插件