Elbaum D, Nagel R L
Prog Clin Biol Res. 1981;55:41-60.
(1) We have observed esterase of Hb A and Hb S. The reaction is consistent with general imidazole catalysis. (2) The rate f p-NPA hydrolysis by Hb A and Hb S is significantly different, probably due to subtle conformational alterations involving beta His 2 and/or its micro-environment in the mutant. (3) During this reaction, irreversible modification of Hb occurs, involving lysines (predominantly Lys beta 82).
(1) 我们观察了Hb A和Hb S的酯酶。该反应与一般的咪唑催化作用一致。(2) Hb A和Hb S对对硝基苯乙酸（p-NPA）的水解速率有显著差异，这可能是由于突变体中涉及β组氨酸2和/或其微环境的细微构象改变所致。(3) 在该反应过程中，Hb发生不可逆修饰，涉及赖氨酸（主要是β82位赖氨酸）。
