Berchtold-Kanz E, Anhut H, Heldt R, Neufang B, Hertting G
Prostaglandins. 1981 Jul;22(1):65-79. doi: 10.1016/0090-6980(81)90054-x.
Seizures were induced in female Wistar rats by electroconvulsive shock (ECS) or administration of pentetrazole (PTZ). Brain content of various prostanoids measured by radioimmunoassay showed time-dependent changes after the induction of convulsions; highest levels were found for PGD2 followed by PGF2 alpha, PGE2, TXB2 and 6-keto-PGF1 alpha. Analysis of the various arachidonic acid metabolites in seven parts of the rat brain dissected according to the method of Glowinski and Iversen revealed the largest increases in hippocampus and cerebral cortex and smaller ones also in hypothalamus and corpus striatum both after ECS and PTZ. The ratios of the different cyclo-oxygenase products remained virtually the same in whole brain as well as in those regions where the formation of prostaglandins was markedly elevated. 15-keto-13,14-dihydro-PGF2 alpha also increased simultaneously in parallel to its parent compound, PGF2 alpha and was detected in significant amounts only in hippocampus and cerebral cortex. However, concentrations of 15-keto-13,14-dihydro-PGF2 alpha in these brain regions as well as in whole brain represented only 3-10% of the amounts found for PGF2 alpha. Thus, the metabolizing enzymes 15-hydroxy-PG-dehydrogenase and delta 13-PG-reductase seem to be of minor importance for the inactivation of prostanoids in brain tissue.
通过电惊厥休克（ECS）或给予戊四氮（PTZ）诱导雌性Wistar大鼠惊厥。通过放射免疫测定法测量的各种前列腺素的脑含量在惊厥诱导后显示出时间依赖性变化；PGD2水平最高，其次是PGF2α、PGE2、TXB2和6-酮-PGF1α。根据Glowinski和Iversen的方法对大鼠脑的七个部分中的各种花生四烯酸代谢产物进行分析，结果显示，在ECS和PTZ处理后，海马体和大脑皮层中的增加最为显著，下丘脑和纹状体中的增加也较小。在全脑以及前列腺素形成明显升高的那些区域，不同环氧化酶产物的比例几乎保持不变。15-酮-13,14-二氢-PGF2α也与其母体化合物PGF2α同时平行增加，并且仅在海马体和大脑皮层中检测到大量该物质。然而，这些脑区以及全脑中15-酮-13,14-二氢-PGF2α的浓度仅占PGF2α含量的3-10%。因此，代谢酶15-羟基-PG脱氢酶和δ13-PG还原酶似乎对脑组织中前列腺素的失活作用较小。
