Nakashima T, Fox S W
Biosystems. 1981;14(2):151-61. doi: 10.1016/0303-2647(81)90064-2.
When lysine-rich proteinoid, which catalyzes the formation of peptides from amino acids and ATP, is complexed with acidic proteinoid to form microspheres of mixed constitution, the normal synthesis by basic proteinoid alone is multiplied several-fold. The product consists not only of small peptides but also of a high-molecular-weight fraction of substituted proteinoid. Suspensions of particles of lysine-rich proteinoid complexed with polyadenylic acid catalyze the synthesis of peptides from each of the amino acids tested with ATP. When equimolar solutions of mixtures of glycine and phenylalanine with ATP are tested in suspensions of complexes of lysine-rich proteinoid and each of various polyribonucleotides, both homopeptides and heteropeptides are produced. Glycylphenylalanine or phenylalanylglycine is the principal product; the preference is related to which polyribonucleotide is in the complex. The rate of conversion of amino acid to peptide is a function of whether ATP is added in a single batch or in repeated amounts adding to the same amount as in the single batch. Related experiments indicate a relatively rapid initial rate of decay of ATP in the system. These results are discussed relative to the mechanisms for continuous generation in modern organisms, as are the results in peptide formation.
当富含赖氨酸的类蛋白质（它能催化氨基酸和ATP形成肽）与酸性类蛋白质复合形成混合结构的微球体时，仅由碱性类蛋白质进行的正常合成会增加几倍。产物不仅包括小肽，还包括高分子量的取代类蛋白质部分。富含赖氨酸的类蛋白质与聚腺苷酸复合的颗粒悬浮液能催化用ATP测试的每种氨基酸合成肽。当用ATP测试甘氨酸和苯丙氨酸混合物的等摩尔溶液在富含赖氨酸的类蛋白质与各种多聚核糖核苷酸各自形成的复合物悬浮液中时，会产生同肽和杂肽。甘氨酰苯丙氨酸或苯丙氨酰甘氨酸是主要产物；这种偏好与复合物中存在哪种多聚核糖核苷酸有关。氨基酸转化为肽的速率取决于ATP是一次性加入还是分多次加入且总量与一次性加入时相同。相关实验表明系统中ATP的初始衰减速率相对较快。这些结果以及肽形成的结果都相对于现代生物体中持续产生的机制进行了讨论。
