Powell T, Sturridge M F, Suvarna S K, Terrar D A, Twist V W
Br Med J (Clin Res Ed). 1981 Oct 17;283(6298):1013-5. doi: 10.1136/bmj.283.6298.1013.
The intricate architecture of heart muscle, comprising irregularly shaped cells which interdigitate in a complex three-dimensional array, has often compromised clear interpretation of experimental data obtained from the whole organ. One approach to minimise some of the difficulties is to use individual muscle cells in suspension, and data have already been reported using myocytes isolated from mammalian ventricles. It is difficult, however, to extrapolate results obtained from animal tissues to situations of medical relevance in man. Intact isolated muscle cells were obtained from human ventricular tissue by modifications of methods used for isolating smooth muscle, atrial, and ventricular tissue from animals. Electrical studies showed that these myocytes had functional characteristics similar to those observed in the whole heart. Such cells will prove a useful preparation for studies on both the mechanisms underlying myocardial performance in normal and diseased states and the response of heart tissue at the cellular level to conditions found during cardiac surgery.
心肌的结构错综复杂，由形状不规则的细胞以复杂的三维阵列相互交错组成，这常常妨碍了对从整个器官获得的实验数据的清晰解读。减少一些困难的一种方法是使用悬浮的单个肌肉细胞，并且已经有使用从哺乳动物心室分离的心肌细胞的相关数据报道。然而，将从动物组织获得的结果外推至与人类医学相关的情况是困难的。通过改进从动物分离平滑肌、心房和心室组织的方法，从人类心室组织中获得了完整的分离肌肉细胞。电学研究表明，这些心肌细胞具有与在整个心脏中观察到的功能特征相似的特征。这些细胞将被证明是研究正常和患病状态下心肌性能的潜在机制以及心脏组织在细胞水平对心脏手术中发现的状况的反应的有用制剂。
