Lefevre P A, Ashby J
Carcinogenesis. 1981;2(9):927-31. doi: 10.1093/carcin/2.9.927.
The effects of the co-mutagen norharman on the mutagenicity of the rodent liver carcinogens 4-dimethyl-aminoazobenzene (DAB) and 3'-methyl-4-dimethyl-aminoazobenzene (3'-MeDAB) have been evaluated using the Ames Salmonella mutation assay. A period of pre-incubation was found to be necessary in order to detect DAB as a mutagen and to increase the initially weak response observed for 3'MeDAB; maximum responses were seen after 1 h pre-incubation in each case. The co-mutagenic properties of norharman were at their maximum for both azo-dyes in the direct plate-incorporation assay. Pre-incubation of either compound with norharman for increasing periods of time resulted in a decrease in potentiation, until after 1 h, an inhibition of mutagenicity was observed.
已使用艾姆斯沙门氏菌突变试验评估了共诱变剂去甲哈尔满对啮齿动物肝脏致癌物4-二甲基氨基偶氮苯（DAB）和3'-甲基-4-二甲基氨基偶氮苯（3'-MeDAB）诱变性的影响。发现预孵育一段时间对于将DAB检测为诱变剂以及增强最初观察到的3'-MeDAB的微弱反应是必要的；在每种情况下，预孵育1小时后观察到最大反应。在直接平板掺入试验中，去甲哈尔满对两种偶氮染料的共诱变特性均达到最大值。将任何一种化合物与去甲哈尔满预孵育更长时间会导致增效作用降低，直到1小时后，观察到诱变性受到抑制。
