通过免疫扩散、免疫渗透电泳和酶联免疫吸附测定法检测和鉴定感染甘蔗中的斐济病病毒。

Detection and identification of FIJI disease virus in infected sugarcane by immunodiffusion, immuno-osmophoretic and enzyme-linked immunosorbent assays.

作者信息

Rohozinski J, Francki R I, Chu P W

出版信息

J Virol Methods. 1981 Oct;3(3):177-86. doi: 10.1016/0166-0934(81)90052-5.

Abstract

Radial double-immunodiffusion, immuno-osmophoretic and enzyme-linked immunosorbent assay (ELISA) methods have been compared for the detection of Fiji disease virus (FDV) in infected sugarcane tissue extracts using an antiserum containing antibodies specific to FDV proteins and ds-RNA. ELISA was the most sensitive of these tests and detected only FDV-specific proteins byt not ds-RNA. Immuno-osmophoretic tests were less sensitive than ELISA but detected both the protein and ds-RNA antigens as distinct precipitin lines. Immunodiffusion tests were much less sensitive for the detection of FDV antigens than either ELISA or immuno-osmophoretic tests. FDV antigens were detected in leaves of virus-infected sugarcane, but only in tissues of the galls which develop in response to infection. Even ELISA failed to detect any antigens in normal tissues adjacent to galls. It is concluded that for the identification to FDV in infected surgarcane, it is necessary to observe galls which can then be tested for the presence of FDV antigens. Immuno-osmophoresis appears to be a satisfactory method for such tests.

摘要

已比较了径向双向免疫扩散、免疫渗透电泳和酶联免疫吸附测定（ELISA）方法，以使用含有针对斐济病病毒（FDV）蛋白和双链RNA的特异性抗体的抗血清，检测感染甘蔗组织提取物中的斐济病病毒（FDV）。ELISA是这些检测中最灵敏的，仅检测到FDV特异性蛋白，而未检测到双链RNA。免疫渗透电泳检测不如ELISA灵敏，但能检测到蛋白质和双链RNA抗原，呈现为明显的沉淀线。免疫扩散检测对FDV抗原的检测灵敏度远低于ELISA或免疫渗透电泳检测。在病毒感染的甘蔗叶片中检测到了FDV抗原，但仅在因感染而形成的瘿组织中检测到。即使是ELISA也未能在瘿附近的正常组织中检测到任何抗原。结论是，为了在受感染的甘蔗中鉴定FDV，有必要观察瘿，然后对其进行FDV抗原检测。免疫渗透电泳似乎是进行此类检测的一种令人满意的方法。