Young C R, Connor G P, Atassi M Z
J Immunogenet. 1981 Oct;8(5):387-94. doi: 10.1111/j.1744-313x.1981.tb00943.x.
We have previously shown that the antibody response to mammalian myoglobins is under genetic control. In the present study we examined antibodies to sperm-whale. Atlantic bottlenosed dolphin, Black Sea dolphin, horse and badger myoglobins, raised in high responder strains of mice, to ascertain whether there is genetic control of antibody affinity to mammalian myoglobins. Using antisera of varying dilutions, the binding to 125I-labelled homologous myoglobins was studied by inhibition with homologous myoglobin over a wide range of inhibitor concentration in a modified Farr essay. The results indicated that there are no large differences between high responder strains of mice in the affinity of antibodies to mammalian myoglobins.
我们之前已经表明，对哺乳动物肌红蛋白的抗体反应受基因控制。在本研究中，我们检测了在高反应性小鼠品系中产生的针对抹香鲸、大西洋宽吻海豚、黑海海豚、马和獾肌红蛋白的抗体，以确定对哺乳动物肌红蛋白的抗体亲和力是否存在基因控制。使用不同稀释度的抗血清，在改良的法尔试验中，通过在广泛的抑制剂浓度范围内用同源肌红蛋白抑制，研究了与125I标记的同源肌红蛋白的结合。结果表明，高反应性小鼠品系对哺乳动物肌红蛋白的抗体亲和力没有很大差异。
