Bagnara G P, Biagini G, Marani M, Bonsi L, Severi B, Valvassori L, Comis M, Nobile F, Iacopino P, Ronco F, Neri A
Acta Haematol. 1981;66(3):174-80. doi: 10.1159/000207116.
T lymphocytic colony formation by peripheral lymphocytes separated by discontinuous albumin gradient centrifugation was evaluated in 8 patients with Philadelphia (Ph1)-positive chronic myeloid leukemia (CML). Colonies were obtained using a liquid-on-agar culture system recently introduced (PHA overlayer-leukocyte feeder layer assay) which has been shown to be simple and reliable. The pattern of colony growth in CML and in normal controls was similar, the peak ranging from the 4th to the 6th day. Also the morphological aspects of colonies did not differ in the two groups. Cells recovered from CML lymphocytic colonies were shown to belong to T cell lineage, as they are able to form spontaneous E-rosettes and to respond to mitogenic stimulation in vitro. In contrast, cells recovered from all other cultured fractions failed to display these properties. Cytogenetic analysis showed that T colony cells were Ph1-negative whereas the chromosome anomaly was found in nonlymphoid colonies of the same patients, thus suggesting a nonclonal origin of T lymphocytes in CML.
对8例费城（Ph1）阳性慢性粒细胞白血病（CML）患者，评估了通过不连续白蛋白梯度离心分离的外周淋巴细胞形成T淋巴细胞集落的情况。采用最近引入的液体覆盖琼脂培养系统（PHA覆盖层-白细胞饲养层试验）获得集落，该系统已被证明简单可靠。CML患者和正常对照的集落生长模式相似，高峰出现在第4至6天。两组集落的形态学特征也无差异。从CML淋巴细胞集落中回收的细胞显示属于T细胞系，因为它们能够形成自发E花环并在体外对有丝分裂刺激作出反应。相比之下，从所有其他培养部分回收的细胞未能表现出这些特性。细胞遗传学分析表明，T集落细胞为Ph1阴性，而在同一患者的非淋巴细胞集落中发现了染色体异常，因此提示CML中T淋巴细胞的起源是非克隆性的。
