Gay C V, Schraer H, Shanabrook V M
Am J Physiol. 1981 Nov;241(5):G382-8. doi: 10.1152/ajpgi.1981.241.5.G382.
Carbonic anhydrase was localized at the ultrastructural level by autoradiography of [3H]acetazolamide in quail gastric mucosa. Grains were associated predominantly with mucin granules at the apical end of surface mucosal cells. Secreted mucin was also labeled. Oxynticopeptic cells of the multilobular glands were not labeled. The pH of fluid secreted by was 7.0-7.4 compared with a pH of 1.3-1.8 of freshly secreted fluid in the gastric lumen. The results of this study suggest that surface mucosal cells, which are strikingly similar in birds, amphibians, and mammals, are the site of acid secretion in the vertebrate stomach.
通过对鹌鹑胃黏膜中[3H]乙酰唑胺进行放射自显影，在超微结构水平上定位了碳酸酐酶。银粒主要与表面黏膜细胞顶端的黏液颗粒相关。分泌的黏液也被标记。多叶腺的泌酸胃酶细胞未被标记。所分泌液体的pH值为7.0 - 7.4，而胃腔内新分泌液体的pH值为1.3 - 1.8。本研究结果表明，在鸟类、两栖动物和哺乳动物中显著相似的表面黏膜细胞是脊椎动物胃中酸分泌的部位。
