噬菌体PBS2诱导的尿嘧啶-DNA糖基化酶抑制剂的特异性

Specificity of the bacteriophage PBS2 induced inhibitor of uracil-DNA glycosylase.

作者信息

Karran P, Cone R, Friedberg E C

出版信息

Biochemistry. 1981 Oct 13;20(21):6092-6. doi: 10.1021/bi00524a027.

Abstract

The purified PBS2 phage-coded inhibitor of uracil-DNA glycosylase (Ura-DNA glycosylase) from Bacillus subtilis has been tested for its ability to inhibit this enzyme isolated from other prokaryotic and from eukaryotic sources. In addition, the inhibitor has been assayed for its effect on DNA glycosylases specific for other base residues in DNA. The data indicate that Ura-DNA glycosylases from a variety of sources are equally sensitive to inhibition by the inhibitor. DNA glycosylases specific for base residues in DNA other than uracil are not inhibited by the PBS2-coded inhibitor.

摘要

已对从枯草芽孢杆菌中纯化得到的PBS2噬菌体编码的尿嘧啶-DNA糖基化酶（Ura-DNA糖基化酶）抑制剂抑制从其他原核生物和真核生物来源分离得到的该酶的能力进行了测试。此外，还测定了该抑制剂对DNA中其他碱基残基特异性的DNA糖基化酶的影响。数据表明，来自多种来源的Ura-DNA糖基化酶对该抑制剂的抑制作用同样敏感。对DNA中除尿嘧啶以外的碱基残基具有特异性的DNA糖基化酶不受PBS2编码的抑制剂的抑制。