Besagni D, Minn A, Gayet J
C R Seances Acad Sci III. 1981 Jul 6;293(1):85-8.
Using a rapid centrifugation-stop procedure, intrasynaptosomal transport of L-glutamine has been measured in conditions allowing the determination of initial rates of this transport. The transport followed saturation kinetics (Km = 0.52 mM; Vmax = 78.24 nmol/min. mg protein), was inhibited at low incubation temperature values and needed a metabolic energy supply. These results demonstrate that L-glutamine is probably transported into synaptic endings via a carrier-mediated mechanism.
采用快速离心停止法，在能够测定L-谷氨酰胺初始转运速率的条件下，对其在突触小体内的转运进行了测量。该转运遵循饱和动力学（Km = 0.52 mM；Vmax = 78.24 nmol/分钟·毫克蛋白），在较低的孵育温度下受到抑制，并且需要代谢能量供应。这些结果表明，L-谷氨酰胺可能是通过载体介导的机制转运到突触末梢的。
