[L-Glutamine transport into synaptosomes isolated from rat brain].

Using a rapid centrifugation-stop procedure, intrasynaptosomal transport of L-glutamine has been measured in conditions allowing the determination of initial rates of this transport. The transport followed saturation kinetics (Km = 0.52 mM; Vmax = 78.24 nmol/min. mg protein), was inhibited at low incubation temperature values and needed a metabolic energy supply. These results demonstrate that L-glutamine is probably transported into synaptic endings via a carrier-mediated mechanism.