钴螯合酶和铁螯合酶的特性。
Properties of the cobaltochelatase and the ferrochelatase.
作者信息
Canepa E T, Llambias E B
出版信息
Enzyme. 1981;26(6):288-95. doi: 10.1159/000459196.
PMID:6796410
Abstract
The formation of cobaltomesoporphyrin and ferromesoporphyrin in the presence of appropriate, chelatases has been studied. The Km value for mesoporphyrin differs for the two activities, although the optimum pH value is 8.1 for both. At high cobalt concentrations a slight non-enzymatic reaction occurs which is not seen with ferrous salts. Both activities are inhibited by EDTA and by sulphhydryl reagents, whereas disulphide reagents inhibit the cobaltochelatase only.
摘要
在适当的螯合酶存在的情况下，钴中卟啉和亚铁中卟啉的形成已经得到研究。两种活性中，中卟啉的米氏常数不同，尽管两者的最适pH值均为8.1。在高钴浓度下会发生轻微的非酶反应，亚铁盐则不会出现这种情况。两种活性均受到EDTA和巯基试剂的抑制，而二硫试剂仅抑制钴螯合酶。
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