渗透力在胞吐作用中的作用：抗利尿激素诱导蟾蜍膀胱融合的研究
Role of osmotic forces in exocytosis: studies of ADH-induced fusion in toad urinary bladder.
作者信息
Kachadorian W A, Muller J, Finkelstein A
出版信息
J Cell Biol. 1981 Nov;91(2 Pt 1):584-8. doi: 10.1083/jcb.91.2.584.
Abstract
Antidiuretic hormone (ADH) treatment of toad urinary bladder activates an exocytotic-like process by which intramembrane particle aggregates are transferred from membranes of elongated cytoplasmic tubules to the luminal-facing plasma membrane. We find that the number of these ADH-induced fusion events, and the number of aggregates appearing in the luminal membrane, are reduced when the luminal bathing medium is made hyperosmotic. As an apparent consequence of the inhibition of their fusion with the luminal membrane, the elongated cytoplasmic tubules become enormously swollen into large, rounded vesicles. These results are consistent with the view that osmotic forces are essential to the basic mechanism of exocytosis.
摘要
抗利尿激素（ADH）对蟾蜍膀胱的处理激活了一个类似胞吐的过程，通过这个过程，膜内颗粒聚集体从细长的细胞质小管膜转移到面向管腔的质膜。我们发现，当管腔灌流介质变为高渗时，这些ADH诱导的融合事件的数量以及出现在管腔膜中的聚集体数量会减少。作为它们与管腔膜融合受到抑制的一个明显结果，细长的细胞质小管会极度肿胀成大的圆形囊泡。这些结果与渗透压对胞吐基本机制至关重要的观点一致。
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