Madhavan M M, Madhavan K
J Embryol Exp Morphol. 1980 Dec;60:1-31.
Mitotic pattern in the different histoblast nests, and the temporal sequence of fusion and differentiation of these nests and spiracular anlagen resulting in the formation of the different regions of the adult abdomen of Drosophila melanogaster were studied by examining whole mount preparations and histological sections of the epidermis from closely timed developmental stages. The relationship between the boundaries of the primary (larval) and secondary (adult) segments was determined by following the points of insertion of the dorsal internal oblique muscles which persist through metamorphosis. These studies indicate that the descendants of the anterior dorsal histoblast nest form the hairy and bristled region of the tergum, while those of the anterior and posterior groups of the posterior dorsal nest give rise to the intersegmental membrane and acrotergite respectively; the ventral histoblast cells give rise to the sternum and pleural region while the spiracular anlage forms the spiracle. These findings confirm and extend the conclusions derived from genetic analysis or after experimental inductions of defects, on the lineage of the various histoblast nests.
通过检查发育阶段紧密相连的表皮整装标本和组织切片，研究了黑腹果蝇不同组织母细胞巢中的有丝分裂模式，以及这些巢与气门原基融合和分化的时间顺序，这些过程导致了成年果蝇腹部不同区域的形成。通过追踪贯穿变态过程的背侧内斜肌的插入点，确定了初级（幼虫）和次级（成虫）节段边界之间的关系。这些研究表明，前背组织母细胞巢的后代形成背板有毛和有刚毛的区域，而后背巢的前后组后代分别形成节间膜和端背片；腹侧组织母细胞产生腹板和胸膜区域，而气门原基形成气门。这些发现证实并扩展了从遗传分析或实验诱导缺陷后得出的关于各种组织母细胞巢谱系的结论。
