用还原活化的丝裂霉素C对小牛胸腺DNA进行烷基化作用。
Alkylation of calf thymus DNA with reductively activated mitomycin C.
作者信息
Hashimoto Y, Shudo K, Okamoto T
出版信息
Nucleic Acids Symp Ser. 1981(10):217-20.
PMID:6796944
Abstract
Mitomycin C (MMC) was catalytically reduced in the presence of a nucleotide or calf thymus DNA. Reaction with 5'-guanylic acid (5'-GMP) gave 1,2-cis-2, 7-diamino-1-(5'-guanylyl) mitosene. Reaction with calf thymus DNA gave modified DNA, which on enzymatic hydrolysis gave two alkylated 5'-deoxyguanylic acid (MG-1 and MG-2) and an alkylated 5'-deoxyadenylic acid (MA). This is the first example of isolation of nucleotides from DNA modified by MMC.
摘要
丝裂霉素C（MMC）在核苷酸或小牛胸腺DNA存在下被催化还原。与5'-鸟苷酸（5'-GMP）反应生成1,2-顺式-2,7-二氨基-1-（5'-鸟苷基）丝裂烯。与小牛胸腺DNA反应得到修饰的DNA，经酶水解后得到两种烷基化的5'-脱氧鸟苷酸（MG-1和MG-2）和一种烷基化的5'-脱氧腺苷酸（MA）。这是从经MMC修饰的DNA中分离核苷酸的首个实例。
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