Strauss R G, Spitzer R E, Stitzel A E, Urmson J R
Transfusion. 1981 Nov-Dec;21(6):693-8. doi: 10.1046/j.1537-2995.1981.21682085757.x.
The granulocyte adherence assay was investigated as a potential in vitro model of filtration leukapheresis to study the effects produced on the complement system by passing anticoagulated blood through columns packed with nylon fibers. Complement activation per se was found not to be a prerequisite for the simple attachment of neutrophils to nylon fibers in this system, because none of the complement components studied were consumed in blood freely flowing through or incubated within adherence columns. Moreover, concentrations of the B antigenic determinant of C3 were unchanged, an indication that this pivotal component had not been cleaved. Although not required for adherence, complement activation is probably initiated by the process of neutrophil attachment, because chemotactic activity was generated. The precise nature of this activity was not identified, but information from previous studies of filtration leukapheresis and granulocyte adherence suggests the possibility of complement origin.
粒细胞黏附试验作为过滤白细胞单采术的一种潜在体外模型进行了研究，以探讨抗凝血液通过填充尼龙纤维的柱体时对补体系统产生的影响。在该系统中，发现补体激活本身并非中性粒细胞简单黏附于尼龙纤维的先决条件，因为在所研究的补体成分中，没有一种在流经黏附柱或在黏附柱内孵育的血液中被消耗。此外，C3的B抗原决定簇浓度未发生变化，这表明这一关键成分未被裂解。虽然黏附不需要补体激活，但补体激活可能是由中性粒细胞黏附过程引发的，因为产生了趋化活性。这种活性的确切性质尚未确定，但先前关于过滤白细胞单采术和粒细胞黏附的研究信息提示了补体来源的可能性。
