[肿瘤细胞和病毒转化细胞中前列腺素E的体外生物合成初步观察]
[Preliminary observations on in vitro biosynthesis of prostaglandins E in tumor cells and virus transformed cells].
作者信息
Capuzzo A, Corallini A, Fabbri E, Ferretti M E, Pareschi M, Tomasi V, Trevisani A
出版信息
Boll Soc Ital Biol Sper. 1981 Oct 30;57(20):2104-10.
Biosynthesis of prostaglandins of E series in BK virus-transformed rabbit-kidney cells (RKBK) and mouse hepatoma cells cultured in vitro with and without exogenous arachidonic acid was determined by radioimmunoassay. Elevated concentrations of prostaglandin E2 were observed in both cell types; moreover, tumor and transformed cells were capable to synthesize higher levels of prostaglandin E2 than normal cells. Mouse hepatoma cells produced more prostaglandins compared to RKBK cells; in addition they seemed less sensitive to inhibition by indomethacin. These data suggest that arachidonic acid metabolism may be another one of the intrinsic biochemical properties that differentiate tumor and virus transformed cells.
采用放射免疫分析法测定了在添加和不添加外源性花生四烯酸的情况下，BK病毒转化的兔肾细胞（RKBK）和体外培养的小鼠肝癌细胞中E系列前列腺素的生物合成。在这两种细胞类型中均观察到前列腺素E2浓度升高；此外，肿瘤细胞和转化细胞能够合成比正常细胞更高水平的前列腺素E2。与RKBK细胞相比，小鼠肝癌细胞产生的前列腺素更多；此外，它们似乎对吲哚美辛的抑制作用不太敏感。这些数据表明，花生四烯酸代谢可能是区分肿瘤细胞和病毒转化细胞的内在生化特性之一。
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